Methods: Blood samples were collected from 5 pediatric patients [13±6 (mean±SD) months, 17±2 LVEF%] with HF before and at 4 hrs, 1, 3, 7, 14 and 30 days after VAD implant. C-miRNA profile at VAD implant and after 30 days was determined by NGS. Modified c-miRNA were validated by qRT-PCR and assessed in blood samples collected during time-course after VAD implant. Putative miRNA targets were selected using miRWalk database. Results: After NGS, a total of 169 c-miRNAs were detected in serum samples of HF pediatric patients. N=13 c-miRNAs were simultaneously modulated at 30 days after VAD implant compared to pre-VAD (Table 1). Among them, only 6 cmiRNAs were confirmed by qRT-PCR (Fig. 1A). Putative targets of selected cmiRNAs seem to be involved with a synergistic effect in the hemostatic process(Fig. 1B).
Circulating microRNA profiling in serum of pediatric patients with heart failure submitted to VAD implant
C Caselli;M G Trivella;L Pitto;M Cabiati;S Del Ry;M Rizzo;R D'Aurizio
2018
Abstract
Methods: Blood samples were collected from 5 pediatric patients [13±6 (mean±SD) months, 17±2 LVEF%] with HF before and at 4 hrs, 1, 3, 7, 14 and 30 days after VAD implant. C-miRNA profile at VAD implant and after 30 days was determined by NGS. Modified c-miRNA were validated by qRT-PCR and assessed in blood samples collected during time-course after VAD implant. Putative miRNA targets were selected using miRWalk database. Results: After NGS, a total of 169 c-miRNAs were detected in serum samples of HF pediatric patients. N=13 c-miRNAs were simultaneously modulated at 30 days after VAD implant compared to pre-VAD (Table 1). Among them, only 6 cmiRNAs were confirmed by qRT-PCR (Fig. 1A). Putative targets of selected cmiRNAs seem to be involved with a synergistic effect in the hemostatic process(Fig. 1B).File | Dimensione | Formato | |
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Descrizione: Circulating microRNA profiling in serum of pediatric patients with heart failure submitted to VAD implant
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