A survey was carried out to check the possible infectivity of soil samples collected from fields where crops were grown which revealed infections by non soilborne viruses. These samples were previously checked to exclude the presence of fungi and/or nematodes known to be virus vectors. Then, they were analyzed either by treatment with 0.01M Tris-HCl buffer, pH 7.6, to attempt the extraction of virus particles, or checking the effects on bait plants. One of this samples, in which tomato seedlings were previously transplanted, after this trial, in presence of tomato roots and other vegetation debris, was mixed with sterile peat and used for transplanting as bait plants Nicotiana benthamiana, N. occidentalis and again tomato. These three hosts revealed presence of viral particles with ultrastructural characteristics typical of closterovirus-like particles. Similar particles were extracted directly from the soil
Si e' indagato sulla possibilita' di conservazione della carica infettiva di terreni contenenti residui di piante infette da virus normalmente ritenuti non trasmissibili attraverso il terreno. I campioni di terreno, esaminati per escludere la presenza di eventuali funghi o nematodi vettori di virus, sono stati sottoposti sia a trattamento con tampone 0.01 M Tris-HCl, pH 7.6, verificando l'estrazione di eventuali particelle virali, sia all'azione di opportune piante esca. Un campione in esame, dopo aver ospitato pomodoro come pianta esca, con residui della coltura e' stato mescolato con torba e sottoposto nuovamente all'azione di pomodoro e di altre piante esca (Nicotiana benthamiana N. occidentalis). Sia in questi ospiti, che nel terreno esaminato direttamente, si e' evidenziata la presenza di particelle virali con caratteristiche ultrastrutturali analoghe a quelle dei closterovirus
AGRIS-FAO dbase: Preliminary observations on the possible soilborne transmission of closterovirus-like particles
Boari A;De Stradis A;
1994
Abstract
A survey was carried out to check the possible infectivity of soil samples collected from fields where crops were grown which revealed infections by non soilborne viruses. These samples were previously checked to exclude the presence of fungi and/or nematodes known to be virus vectors. Then, they were analyzed either by treatment with 0.01M Tris-HCl buffer, pH 7.6, to attempt the extraction of virus particles, or checking the effects on bait plants. One of this samples, in which tomato seedlings were previously transplanted, after this trial, in presence of tomato roots and other vegetation debris, was mixed with sterile peat and used for transplanting as bait plants Nicotiana benthamiana, N. occidentalis and again tomato. These three hosts revealed presence of viral particles with ultrastructural characteristics typical of closterovirus-like particles. Similar particles were extracted directly from the soilI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.