A survey was carried out to check the possibleinfectivity of soil samples collected from fields wherecrops were grown which revealed infections by nonsoilborne viruses. These samples were previouslychecked to exclude the presence of fungi and/ornematodes known to be virus vectors. Then, they wereanalyzed either by treatment with 0.01M Tris-HCl buffer,pH 7.6, to attempt the extraction of virus particles, orchecking the effects on bait plants. One of thissamples, in which tomato seedlings were previouslytransplanted, after this trial, in presence of tomatoroots and other vegetation debris, was mixed withsterile peat and used for transplanting as bait plantsNicotiana benthamiana, N. occidentalis and againtomato. These three hosts revealed presence of viralparticles with ultrastructural characteristics typical ofclosterovirus-like particles. Similar particles wereextracted directly from the soil
Si e' indagato sulla possibilita' di conservazione dellacarica infettiva di terreni contenenti residui di pianteinfette da virus normalmente ritenuti non trasmissibiliattraverso il terreno. I campioni di terreno, esaminatiper escludere la presenza di eventuali funghi onematodi vettori di virus, sono stati sottoposti sia atrattamento con tampone 0.01 M Tris-HCl, pH 7.6,verificando l'estrazione di eventuali particelle virali, sia all'azione di opportune piante esca. Un campione inesame, dopo aver ospitato pomodoro come piantaesca, con residui della coltura e' stato mescolato contorba e sottoposto nuovamente all'azione di pomodoroe di altre piante esca (Nicotiana benthamiana N.occidentalis). Sia in questi ospiti, che nel terrenoesaminato direttamente, si e' evidenziata la presenzadi particelle virali con caratteristiche ultrastrutturalianaloghe a quelle dei closterovirus
AGRIS-FAO dbase: Preliminary observations on the possible soilborne transmission of closterovirus-like particles
Boari A;De Stradis A;
1994
Abstract
A survey was carried out to check the possibleinfectivity of soil samples collected from fields wherecrops were grown which revealed infections by nonsoilborne viruses. These samples were previouslychecked to exclude the presence of fungi and/ornematodes known to be virus vectors. Then, they wereanalyzed either by treatment with 0.01M Tris-HCl buffer,pH 7.6, to attempt the extraction of virus particles, orchecking the effects on bait plants. One of thissamples, in which tomato seedlings were previouslytransplanted, after this trial, in presence of tomatoroots and other vegetation debris, was mixed withsterile peat and used for transplanting as bait plantsNicotiana benthamiana, N. occidentalis and againtomato. These three hosts revealed presence of viralparticles with ultrastructural characteristics typical ofclosterovirus-like particles. Similar particles wereextracted directly from the soil| File | Dimensione | Formato | |
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