Eucaryotic organisms depend on networks of gene regulatory pathways. Small RNAs (sRNAs), are key components of these networks. sRNAs are short (21-24 nt in length), endogenously expressed, and are processed from double stranded (ds)RNAs or dsRNA-like precursors. In both plants and animals, sRNAs exert their functions upon incorporation into ribonucleoprotein silencing complexes and through their baseparing capacity. They are implicated in a variety of processes, including post-transcriptional regulation of mRNA. RNA silencing in plants can function as a defence mechanism against invading viruses. RNA silencing-based antiviral defence entails the production of virus-derived small interfering RNAs, which guide specific antiviral effector complexes to inactivate viral genomes. During the CNR-MTA joint project 2010-2012 we have investigated some aspects of the RNA silencing which are involved in antiviral mechanisms and in plant development. The main results acquired are summarised as follow:
Nelle piante, i piccoli RNA interferenti regolatori (miRNAs e siRNAs) di lunghezza di 21-24 nucleotidi sono prodotti da specifiche ribonucleasi (DICER) aventi come substrato RNA precursori a doppia elica. I miRNA ed i siRNAs di origine endogena, tipicamente attivano specifici complessi proteici e riconoscono, per complementarieta, Le molecole di mRNAs bersaglio provocandone una sotto-regolazione post-trascrizionale. Gli RNA a doppia elica di origine virale, sono altresi substrato dei DICERs ed inducono la produzione siRNAs virali (vsiRNAs). I vsiRNAs possono guidare la disattivazione degli RNA virali analogamente alIa regolazione post-trascrizionale mediata dei miRNAs. Durante il periodo di collaborazione bilaterale CNR-MTA 2010-2012, sono stati congiuntamente investigati alcuni aspetti molecolari regolatori del silenziamento genico dell'RNA I coinvolti nello sviluppo e differenziazione delle piante e nella difesa antivirale.?
Roles and regulalion of small non-coding RNAs in differentiation and virus/host interactions in plants Rapporto finale Progetto di Ricerca Bilaterale CNR-MTA (Accademia Ungherese delle Scienze)
Pantaleo V
2012
Abstract
Eucaryotic organisms depend on networks of gene regulatory pathways. Small RNAs (sRNAs), are key components of these networks. sRNAs are short (21-24 nt in length), endogenously expressed, and are processed from double stranded (ds)RNAs or dsRNA-like precursors. In both plants and animals, sRNAs exert their functions upon incorporation into ribonucleoprotein silencing complexes and through their baseparing capacity. They are implicated in a variety of processes, including post-transcriptional regulation of mRNA. RNA silencing in plants can function as a defence mechanism against invading viruses. RNA silencing-based antiviral defence entails the production of virus-derived small interfering RNAs, which guide specific antiviral effector complexes to inactivate viral genomes. During the CNR-MTA joint project 2010-2012 we have investigated some aspects of the RNA silencing which are involved in antiviral mechanisms and in plant development. The main results acquired are summarised as follow:I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
