Ce mémoire représente le travail de neuf mois de collaboration entre le « Surface Science laboratory » de « European Synchrotron Radiation Facility » de Grenoble e l'Institute de Biophysique Unité Opérationnelle de Palerme (Italie) du « Consiglio Nazionale delle Ricerche ».Le mémoire présenté a la mission de projeter et optimiser un nouveau prototype de instrumentation scientifique nommé, « Cooling mode - Atomic force microscope », un microscope a force atomique pensé pour étudier un systême biologique vivant dans un liquide.Dans le premier chapitre a été présentée la microscopie atomique, les instrumentations et les techniques utilisées. La présentation de la technique « Cooling mode », de ces caractéristique et similitude avec une autre méthode de fonctionnement, a été présentée dans le second chapitre. Dans ce chapitre a été aussi présenté le premier prototype de microscope CM-AFM, projeté par le Dr. Michal Hrouzek.Le dernier chapitre, présente le travail de développement de la nouvelle version du microscope, améliorée dans la prestation mai aussi dans l'ergonomie. Compte tenue des données obtenues par l'expérimentation du premier prototype, il à été aussi modifié en fonction des observations fournies par les potentiels utilisateurs. Enfin, en conclusion du travail de développement tout au long de cette période du mémoire, ont été formulées plusieurs idées sur l'état du projet et sur les évolutions dans le futur de cette technique d'exploration de l'infiniment petit, dans laquelle a été prévu l'utilisation dans des domaines différentes comme par exemple la chirurgie et l'analyse alimentaire.
This document will present the work done during part of the collaboration between the Surface Science Laboratory of the European Synchrotron Radiation Facility in Grenoble and the Institute of Biophysics Unit of Palermo of the National Research Council (IBF-CNR).The argument presented here has the proposal to redesign and optimize a new prototype scientific instrumentation called "Cooling Mode - Atomic Force Microscope" (CM-AFM), an atomic force microscope designed for the study of biological systems living in a liquid environment .In the first chapter is presented atomic microscopy, including tools and techniques used to the developing of these instruments. In this chapter is introduced the cantilever, defining the mechanical characteristics.The second chapter describes the "Cooling mode" measuring technique, its peculiarities and similarities with other modes of operation, and is also presented the first prototype of the CM-AFM microscope, designed and assembled by Dr. Michal Hrouzek.The last chapter introduces the effort and the results of designing a new version of the microscope, improved not only in performance but also in the ergonomics. At this point, feedbacks and impression from potential users and data from the trial version of the instrument are taken into account.At the end of this work, some observations were made, on the current status of the project and potential future developments of the technique of small infinitely exploration, which forecast a use in areas apparently far, i.e. as surgery and control food.
Analyse modale, thermique structural et optimisation appliquée à un microscope à force atomique / D'Anca, Fabio. - ELETTRONICO. - (2010 Jul 08).
Analyse modale, thermique structural et optimisation appliquée à un microscope à force atomique
Fabio D'Anca
2010
Abstract
This document will present the work done during part of the collaboration between the Surface Science Laboratory of the European Synchrotron Radiation Facility in Grenoble and the Institute of Biophysics Unit of Palermo of the National Research Council (IBF-CNR).The argument presented here has the proposal to redesign and optimize a new prototype scientific instrumentation called "Cooling Mode - Atomic Force Microscope" (CM-AFM), an atomic force microscope designed for the study of biological systems living in a liquid environment .In the first chapter is presented atomic microscopy, including tools and techniques used to the developing of these instruments. In this chapter is introduced the cantilever, defining the mechanical characteristics.The second chapter describes the "Cooling mode" measuring technique, its peculiarities and similarities with other modes of operation, and is also presented the first prototype of the CM-AFM microscope, designed and assembled by Dr. Michal Hrouzek.The last chapter introduces the effort and the results of designing a new version of the microscope, improved not only in performance but also in the ergonomics. At this point, feedbacks and impression from potential users and data from the trial version of the instrument are taken into account.At the end of this work, some observations were made, on the current status of the project and potential future developments of the technique of small infinitely exploration, which forecast a use in areas apparently far, i.e. as surgery and control food.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.