INSILC D3.4 - Cell-based experimental Lab Test Findings

This Deliverable reports the activity of Task 3.3: "In vitro models and molecular pathways of thrombosis and restenosis (protein)". This task is devoted to obtain a panel of molecular markers potentially involved in restenosis and in-stent thrombosis of drug-eluting Bioresorbable Vascular Scaffold (BVS) and to supply it for the development of the silico module implemented in Work Package 6. In order to achieve this objective, gene expression profile is obtained in cultured vascular wall cells, namely human coronary artery endothelial cells (HCAECs) and human coronary artery smooth muscle cells (HCASMCs) submitted to static or dynamic conditions (corresponding to shear stress values of 1 and 20 dyne/cm2) in absence or in presence of a BVS - a PLLA prototype provided by Boston Scientific Corporation - and at a constant drug (Everolimus) concentration: Ibidi µ-Slide systems were adopted as laminar flow 2D bioreactors to evaluate the impact of hemodynamic forces in the presence of drug-eluting BVS on gene expression in human vascular cells.