In situ hybridization techniques and conventional/epifluorescence microscopic analysis have been applied for the biomolecular characterization of mixed biomass occurring in the biological units of the technologies investigated within WP2. In particular, MBR (IRSA-CNR, Bari) and BIOZO-DETOX (NIVA) systems were monitored by FISH (Fluorescence In Situ Hybridization) for determining the abundance and the composition of the microbial communities degrading pharmaceutical and landfill leachate wastewaters respectively. The analysed samples from MBR reactor treating pharmaceutical wastewater showed the occurrence of highly selected mixed cultures mainly composed by a filamentous bacterium known as Nostocoida limicola (belonging to Alphaproteobacteria), previously found as dominant filament causing bulking problems in industrial wastewater systems due to its capability to store high amount of carbon as polyhydroxyalkanoates under aerobic conditions. A negligible effect on biomass composition was further observed after the adoption of the AOP processes. Besides the biomolecular characterization, a set of chemical - physical analyses were carried out on MBR sludge samples, before and after the integration of the advanced oxidation process (UV + H2O2). The selected parameters were: Total and Volatile Solids, soluble COD, Dissolved Oxygen, Capillary Suction Time and pH. Moreover the Heavy Metals (class of pollutants inserted on the 3rd Draft-Working Document on Sludge, 2000) concentration of the MBR sludge samples was measured to assess the sludge quality for agricultural use. The biomolecular characterization of the biofilm growing in BIOZO-DETOX systems was mainly focused on the estimation of the nitrifying and denitrifying microbial populations. Some differences in terms of microbial activity were also observed among reactors (six in-series reactors, three anoxic and three aerated respectively) due to the differently applied contaminant loading conditions and carbon substrate availability.
Deliverable D2.13 Report on physical, chemical and microbiological characteristics of the residues (biomass)
CM Braguglia;M De Sanctis;A Gianico;G Mininni;S Rossetti
2011
Abstract
In situ hybridization techniques and conventional/epifluorescence microscopic analysis have been applied for the biomolecular characterization of mixed biomass occurring in the biological units of the technologies investigated within WP2. In particular, MBR (IRSA-CNR, Bari) and BIOZO-DETOX (NIVA) systems were monitored by FISH (Fluorescence In Situ Hybridization) for determining the abundance and the composition of the microbial communities degrading pharmaceutical and landfill leachate wastewaters respectively. The analysed samples from MBR reactor treating pharmaceutical wastewater showed the occurrence of highly selected mixed cultures mainly composed by a filamentous bacterium known as Nostocoida limicola (belonging to Alphaproteobacteria), previously found as dominant filament causing bulking problems in industrial wastewater systems due to its capability to store high amount of carbon as polyhydroxyalkanoates under aerobic conditions. A negligible effect on biomass composition was further observed after the adoption of the AOP processes. Besides the biomolecular characterization, a set of chemical - physical analyses were carried out on MBR sludge samples, before and after the integration of the advanced oxidation process (UV + H2O2). The selected parameters were: Total and Volatile Solids, soluble COD, Dissolved Oxygen, Capillary Suction Time and pH. Moreover the Heavy Metals (class of pollutants inserted on the 3rd Draft-Working Document on Sludge, 2000) concentration of the MBR sludge samples was measured to assess the sludge quality for agricultural use. The biomolecular characterization of the biofilm growing in BIOZO-DETOX systems was mainly focused on the estimation of the nitrifying and denitrifying microbial populations. Some differences in terms of microbial activity were also observed among reactors (six in-series reactors, three anoxic and three aerated respectively) due to the differently applied contaminant loading conditions and carbon substrate availability.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
