EZcount an All-in-one Software for microRNA Expression Quantification from NGS Sequencing data

MicroRNAs (miRNAs) are short endogenous molecules of RNA that influence cell regulation by suppressing genes. Their ubiquity throughout the branches of the tree of life has suggested their central role in many cellular functions. Nowadays, several personalized medicine applications leverage on miRNAs as biomarkers for diagnoses, prognoses, and drug response prediction. The increasing ease of sequencing miRNAs contrasts with the difficulty of accurately quantify their concentration. The use of general purpose aligners is only a partial solution as they have limited possibility to accurately solve ambiguous mapping due to the short length of these sequences.