To investigate the role of catalase and superoxide dismutase (SOD) in the acetic acid (AA) induced yeast programmed cell death (AA-PCD), we compared Saccharomyces cerevisiae cells (C-Y) and cells individually over-expressing catalase T (CTT1-Y) and Cu,Zn-SOD (SOD1-Y) with respect to cell survival, hydrogen peroxide (H(2)O(2)) levels and enzyme activity as measured up to 200min after AA treatment. AA-PCD does not occur in CTT1-Y, where H(2)O(2) levels were lower than in C-Y and the over-expressed catalase activity decreased with time. In SOD1-Y, AA-PCD was exacerbated; high H(2)O(2) levels were found, SOD activity increased early, remaining constant en route to AA-PCD, but catalase activity was strongly reduced.
Catalase T and Cu,Zn-superoxide dismutase in the acetic acid-induced programmed cell death in Saccharomyces cerevisiae.
Guaragnella N;Giannattasio S;Marra E;
2008
Abstract
To investigate the role of catalase and superoxide dismutase (SOD) in the acetic acid (AA) induced yeast programmed cell death (AA-PCD), we compared Saccharomyces cerevisiae cells (C-Y) and cells individually over-expressing catalase T (CTT1-Y) and Cu,Zn-SOD (SOD1-Y) with respect to cell survival, hydrogen peroxide (H(2)O(2)) levels and enzyme activity as measured up to 200min after AA treatment. AA-PCD does not occur in CTT1-Y, where H(2)O(2) levels were lower than in C-Y and the over-expressed catalase activity decreased with time. In SOD1-Y, AA-PCD was exacerbated; high H(2)O(2) levels were found, SOD activity increased early, remaining constant en route to AA-PCD, but catalase activity was strongly reduced.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
