A one-pot synthesis of homodimeric proteins is described. The synthetic strategy is based on a double expressed protein ligation reaction between thioester peptides and a new bis-cysteinyl linker. The protocol was also applied to the synthesis of heterodimers.
Semisynthesis of Dimeric Proteins by Expressed Protein Ligation
D'Andrea L D;
2008
Abstract
A one-pot synthesis of homodimeric proteins is described. The synthetic strategy is based on a double expressed protein ligation reaction between thioester peptides and a new bis-cysteinyl linker. The protocol was also applied to the synthesis of heterodimers.File in questo prodotto:
| File | Dimensione | Formato | |
|---|---|---|---|
|
prod_13915-doc_21268.pdf
non disponibili
Descrizione: Articolo su rivista
Dimensione
385.37 kB
Formato
Adobe PDF
|
385.37 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
