Drought is one of the most important environmental stress factors limiting wood production of forest trees. The physiological mechanisms involved in plant water-stress adaptation are associated to changes in genes expression. These genes are involved in osmotic adjustment, cellular protection, damages limitation etc.... and were mainly studied in model species and crop plants. Such investigation remains in its infancy in trees. The aim of our work was to study the effect of a water stress followed by a rehydration period in a woody plant model: Populus x canescens (Populus tremula x Populus alba (clone 717-1B4)). The identification of proteins involved in this process will enable us to understand how these long lived organisms respond to drought and eventually derived diagnostic markers for genetic resources management such as improving the drought tolerance of clones of interest. Populus x canescens cuttings were grown in controlled conditions. The stress intensity was estimated by measuring predawn leaf water potential, leaf relative water content, leaf growth, leaf conductance and maximum photosynthesis. Proteome analysis was performed using 2D-PAGE. Proteins were extracted from leaves and cambium tissues isolated from control plants (-0.4MPa), stressed plants (-1.6MPa) and rehydrated plants (- 0.4MPa). Twenty five differentially expressed proteins were identified and for three of them tandem MS data allows their function to be determined. These proteins correspond to a glutathione s-transferase, an oxygenevolving enhancer protein 2 chloroplast precursor, and a 3,4-dihydroxy-2-butanone kinase.
Effect of drought stress followed by a rewatering period on physiological parameters and the proteome of Populus x canescens (clone 717-1B4)
Giovannelli A;
2005
Abstract
Drought is one of the most important environmental stress factors limiting wood production of forest trees. The physiological mechanisms involved in plant water-stress adaptation are associated to changes in genes expression. These genes are involved in osmotic adjustment, cellular protection, damages limitation etc.... and were mainly studied in model species and crop plants. Such investigation remains in its infancy in trees. The aim of our work was to study the effect of a water stress followed by a rehydration period in a woody plant model: Populus x canescens (Populus tremula x Populus alba (clone 717-1B4)). The identification of proteins involved in this process will enable us to understand how these long lived organisms respond to drought and eventually derived diagnostic markers for genetic resources management such as improving the drought tolerance of clones of interest. Populus x canescens cuttings were grown in controlled conditions. The stress intensity was estimated by measuring predawn leaf water potential, leaf relative water content, leaf growth, leaf conductance and maximum photosynthesis. Proteome analysis was performed using 2D-PAGE. Proteins were extracted from leaves and cambium tissues isolated from control plants (-0.4MPa), stressed plants (-1.6MPa) and rehydrated plants (- 0.4MPa). Twenty five differentially expressed proteins were identified and for three of them tandem MS data allows their function to be determined. These proteins correspond to a glutathione s-transferase, an oxygenevolving enhancer protein 2 chloroplast precursor, and a 3,4-dihydroxy-2-butanone kinase.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
