We show a novel solid state optical detection platform, integrated in a plastic biochip, based on colloidal nanocrystal FRET donors. The approach exploits a 'smart' polymeric layer with both optical and biorecognition properties that allows real-time monitoring of bionrolecular interactions and quantitative analyses of real-time PCR. The proposed strategy, demonstrated here for DNA detection, may open interesting perspectives for a wide range of applications, such as for proteomic studies.
Real-Time PCR in a Plastic Chip Based on Solid State FRET
Sabella S;Rinaldi R;Pompa PP
2008
Abstract
We show a novel solid state optical detection platform, integrated in a plastic biochip, based on colloidal nanocrystal FRET donors. The approach exploits a 'smart' polymeric layer with both optical and biorecognition properties that allows real-time monitoring of bionrolecular interactions and quantitative analyses of real-time PCR. The proposed strategy, demonstrated here for DNA detection, may open interesting perspectives for a wide range of applications, such as for proteomic studies.File in questo prodotto:
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