We demonstrate the possibility of using x-ray photoemission spectroscopy (XPS) to distinguish between the chemical properties of the neuron membrane and of the cytoplasm in brain cells. This was possible by analysing cells de-capped by using a newly developed technique. The XPS spectra clearly show chemical differences between the cell cytoplasm and the membrane, for example between nitrogen in and , which are characteristic of the proteins and membrane phospholipids, respectively. A preliminary (and not conclusive) interpretation of all the observed spectroscopic features is given.
photoemission analysis of chemical differences between the membrane and cytoplasm of neuronal cells
1997
Abstract
We demonstrate the possibility of using x-ray photoemission spectroscopy (XPS) to distinguish between the chemical properties of the neuron membrane and of the cytoplasm in brain cells. This was possible by analysing cells de-capped by using a newly developed technique. The XPS spectra clearly show chemical differences between the cell cytoplasm and the membrane, for example between nitrogen in and , which are characteristic of the proteins and membrane phospholipids, respectively. A preliminary (and not conclusive) interpretation of all the observed spectroscopic features is given.File in questo prodotto:
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