The thermostable beta-D-glycosidase from the archaeon Sulfolobus solfataricus was used for the glucosylation of secondary alcohols at high organic solvent concentrations. Alkyl glucoside products were analysed by one- and two-dimensional NMR. Using a range of racemic secondary alcohols and various hydrophilic organic solvents, small but significant substrate- and solvent-dependent changes in the R/S ratios of alkyl glucoside products were detected. The anomeric specificity of the enzyme was apparently affected by butan-2-one. A possible mechanistic model for solvent-induced changes at the active site is presented.
Sulfolobus solfataricus beta-glycosidase catalysed synthesis of sugar alchol conjugates in the presence of organic solvents.
Nucci R;
2000
Abstract
The thermostable beta-D-glycosidase from the archaeon Sulfolobus solfataricus was used for the glucosylation of secondary alcohols at high organic solvent concentrations. Alkyl glucoside products were analysed by one- and two-dimensional NMR. Using a range of racemic secondary alcohols and various hydrophilic organic solvents, small but significant substrate- and solvent-dependent changes in the R/S ratios of alkyl glucoside products were detected. The anomeric specificity of the enzyme was apparently affected by butan-2-one. A possible mechanistic model for solvent-induced changes at the active site is presented.File in questo prodotto:
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