The activities of some enzymes with "antioxidant extracting" properties, namely ascorbate peroxidase (APX, E.C. 1.11.1.11), catalase (CAT, E.C. 1.11.1.6) and superoxide dismutase (SOD, E.C. 1.15.1.1) were evaluated, together with the vitamin C content that may contribute to enhance the antioxidant pattern of tomato fruits and act against browning reactions due to polyphenol oxidase (PPO, E.C. 1.14.18.1) which, together with pectinase, pectin methylesterase (PME, E.C. 3.1.1.11) and polygalacturonase (PG, EC 3.2.1.15) responsible for cell wall softening, negatively affect their shelf life. Three different long-storage tomato genotypes ('Montallegro', 'Filicudi', 'Principe Borghese'), were tested in comparison with the widespread hybrid 'Brigade' cultivated in dry conditions in typical semi-arid Mediterranean environment (Sicily, Italy). The positive compliance between results of enzymes activities and the parameters evidenced the strategic role of enzymatic investigation which could represent a useful tool for the selection of long-storage tomato ecotypes present in Sicily, much requested by the market due to their storage properties and nutraceutical quality.
Sono stati valutati 3 genotipi di pomodoro da serbo ('Montallegro', 'Filicudi', 'Principe Borghese'), coltivati "in seccagno" e confrontati con l'ibrido commerciale 'Brigade' di pomodoro da industria, testando gli enzimi ad azione "elicitaria antiossidante", ascorbato perossidasi, catalasi e superossido dismutasi, insieme con il contenuto di vitamina C che contrasta l'imbrunimento da polifenolossidasi, nonché le attività pectinmetilesterasi e poligalatturonasi, responsabili del rammollimento dei frutti. La buona rispondenza tra i risultati delle attività enzimatiche ed i parametri a supporto, evidenziava il ruolo strategico dell'indagine enzimatica per la selezione degli ecotipi di pomodoro da serbo presenti in Sicilia.
Profilo enzimatico di alcune accessioni di pomodoro da serbo coltivate in Sicilia
Patanè C;Di Silvestro I;
2013
Abstract
The activities of some enzymes with "antioxidant extracting" properties, namely ascorbate peroxidase (APX, E.C. 1.11.1.11), catalase (CAT, E.C. 1.11.1.6) and superoxide dismutase (SOD, E.C. 1.15.1.1) were evaluated, together with the vitamin C content that may contribute to enhance the antioxidant pattern of tomato fruits and act against browning reactions due to polyphenol oxidase (PPO, E.C. 1.14.18.1) which, together with pectinase, pectin methylesterase (PME, E.C. 3.1.1.11) and polygalacturonase (PG, EC 3.2.1.15) responsible for cell wall softening, negatively affect their shelf life. Three different long-storage tomato genotypes ('Montallegro', 'Filicudi', 'Principe Borghese'), were tested in comparison with the widespread hybrid 'Brigade' cultivated in dry conditions in typical semi-arid Mediterranean environment (Sicily, Italy). The positive compliance between results of enzymes activities and the parameters evidenced the strategic role of enzymatic investigation which could represent a useful tool for the selection of long-storage tomato ecotypes present in Sicily, much requested by the market due to their storage properties and nutraceutical quality.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.


