The tomato yellow leaf curl disease (TYLCD) has been known for many years. The cause was, premature but with commendable intuition, put down to an entity named Tomato yellow leaf curl virus (TYLCV) (Cohen and Nitzany, 1966) although the viral etiology was recognized only in the late 1970s, and a virus with geminate morphology detected even later. Electron microscopic (EM) observations of thin sections from TYLCV-infected tomato leaves indicated that geminate particles were located in the nuclei of phloem parenchyma cells (Russo et al., 1980; Cherif & Russo, 1983), with intranuclear occurrence of fibrillar rings and small virus-like particles like those in the new virus group named "geminiviruses" (Goodman, 1981). In those times EM was therefore the only possible way to detect TYLCV. However, what are considered "detection methods" for this virus complex had to wait for isolation of viral particles and demonstration that they are the causal agent of TYLCD. The virus was first isolated and purified in 1988 (Czosnek et al., 1988), and its association with the disease was demonstrated by membrane feeding of the whitefly vector on purified virus preparations. Since then, several detection methods for what is now recognized as a virus complex have been developed, both for mass screening and for more specific characterization. In this review only methods for mass screening will be discussed, omitting specific applications, such as in situ hybridization and immuno-enzymatic methods for light or electron microscopy.

Detection methods for TYLCV and TYLCSV

Accotto;GP;Noris;
2007

Abstract

The tomato yellow leaf curl disease (TYLCD) has been known for many years. The cause was, premature but with commendable intuition, put down to an entity named Tomato yellow leaf curl virus (TYLCV) (Cohen and Nitzany, 1966) although the viral etiology was recognized only in the late 1970s, and a virus with geminate morphology detected even later. Electron microscopic (EM) observations of thin sections from TYLCV-infected tomato leaves indicated that geminate particles were located in the nuclei of phloem parenchyma cells (Russo et al., 1980; Cherif & Russo, 1983), with intranuclear occurrence of fibrillar rings and small virus-like particles like those in the new virus group named "geminiviruses" (Goodman, 1981). In those times EM was therefore the only possible way to detect TYLCV. However, what are considered "detection methods" for this virus complex had to wait for isolation of viral particles and demonstration that they are the causal agent of TYLCD. The virus was first isolated and purified in 1988 (Czosnek et al., 1988), and its association with the disease was demonstrated by membrane feeding of the whitefly vector on purified virus preparations. Since then, several detection methods for what is now recognized as a virus complex have been developed, both for mass screening and for more specific characterization. In this review only methods for mass screening will be discussed, omitting specific applications, such as in situ hybridization and immuno-enzymatic methods for light or electron microscopy.
2007
VIROLOGIA VEGETALE
978-1-4020-4769-5
TYLCSV
TYLCV
Geminivirus
diagnosis
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.14243/141180
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