We describe an additional HLA-Cw*02 variant, HLA-Cw*0208, which has been identified in a renal transplant recipient of Caucasian origin (Italy). After performing preliminary serological typing, we analyzed exons 2 and 3 of the HLA-C locus polymorphism by cloning the amplified DNA and using a sequence-based typing method. The new allele differs from Cw*020202 by one nucleotide substitution at nucleotide 61 (G→ A) of exon 2, which translates to a difference of one amino acid at residue 21 (His→ Arg) of the HLA-C heavy chain. We propose that Cw*0208 was generated by a random point mutation in codon 21 from the Cw*020202 allele, or through gene conversion of Cw*020202 with another allele, probably the Cw*1205 and Cw*1602 alleles.
Characterization of a novel HLA-Cw*02 variant, Cw*0208' in a Caucasian individual
Canossi A
Writing – Original Draft Preparation
;Aureli AFormal Analysis
;Piancatelli DFormal Analysis
;Liberatore GMethodology
;Del Beato TMethodology
;Ozzella GFormal Analysis
;Piazza AWriting – Review & Editing
;
2005
Abstract
We describe an additional HLA-Cw*02 variant, HLA-Cw*0208, which has been identified in a renal transplant recipient of Caucasian origin (Italy). After performing preliminary serological typing, we analyzed exons 2 and 3 of the HLA-C locus polymorphism by cloning the amplified DNA and using a sequence-based typing method. The new allele differs from Cw*020202 by one nucleotide substitution at nucleotide 61 (G→ A) of exon 2, which translates to a difference of one amino acid at residue 21 (His→ Arg) of the HLA-C heavy chain. We propose that Cw*0208 was generated by a random point mutation in codon 21 from the Cw*020202 allele, or through gene conversion of Cw*020202 with another allele, probably the Cw*1205 and Cw*1602 alleles.File | Dimensione | Formato | |
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