1a,25-dihydroxy vitamin D3 [1a,25(OH)2D3], the hormonal form of vitamin D3 that is essential for the maintenance of calcium-phosphorus homeostasisto in birds and mammals, has been found in several plants. In order to study the metabolic role of 1a,25(OH)2D3 in plant cells, we monitored the changes of cellular levels of 1a,25(OH)2D3 in cell suspension cultures of Solanum malacoxylon Sendt. under different experimental conditions. We also measured the levels of cholesterol, the assumed precursor of vitamin D3 , and other end-product sterols such as b-sitosterol, stigmasterol and campesterol. It was found that when the cells are incubated in a calcium deprived medium, thelevel of 1a,25(OH) 2D3 increases markedly within a few hours. Treatment with methyl jasmonate (MeJ), a linolenic acid-derived compound that accumulates during wounding stress, was found to reduce the cellular level of 1a,25 (OH)2D3 and to promote its secretion into the culture medium. After treatment with cellulysin, a cellulase of fungal origin, the metabolite was no longer detectable, either in the medium or in the cells. Calcium deprivation in the medium and treatment with elicitors MeJ and cellulase lowered the cellular level of all the sterols tested. The possible role of 1a,25(OH)2D3 is discussed in the light of these results.
Effect of elicitors and Ca++ deprivation on the levels of sterols and 1alpha, 25-dihydroxy vitamin D3 in cell cultures of Solanum malacoxylon
2002
Abstract
1a,25-dihydroxy vitamin D3 [1a,25(OH)2D3], the hormonal form of vitamin D3 that is essential for the maintenance of calcium-phosphorus homeostasisto in birds and mammals, has been found in several plants. In order to study the metabolic role of 1a,25(OH)2D3 in plant cells, we monitored the changes of cellular levels of 1a,25(OH)2D3 in cell suspension cultures of Solanum malacoxylon Sendt. under different experimental conditions. We also measured the levels of cholesterol, the assumed precursor of vitamin D3 , and other end-product sterols such as b-sitosterol, stigmasterol and campesterol. It was found that when the cells are incubated in a calcium deprived medium, thelevel of 1a,25(OH) 2D3 increases markedly within a few hours. Treatment with methyl jasmonate (MeJ), a linolenic acid-derived compound that accumulates during wounding stress, was found to reduce the cellular level of 1a,25 (OH)2D3 and to promote its secretion into the culture medium. After treatment with cellulysin, a cellulase of fungal origin, the metabolite was no longer detectable, either in the medium or in the cells. Calcium deprivation in the medium and treatment with elicitors MeJ and cellulase lowered the cellular level of all the sterols tested. The possible role of 1a,25(OH)2D3 is discussed in the light of these results.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
