In the AFLP technique has been proposed as a valuable tool for genetic mapping in plant species. In the present contribution, the application of AFLP was investigated for the generation of markers useful for genetic mapping in chickpea. A total of 48 primer combinations were tested in Cicer arietinum ICC 4958, susceptible to the pathogen Fusarium oxysporum f.sp. ciceri and in C. reticu-latum PI 489777, resistant to the same fungus. The electrophoretic pattern showed an average of 38 bands for each primer combination and the polymorphic fragments between the two species were comprised between 1 and 15 fragments, Twelve primer combinations did not provide interpretable results. The six most polymorphic primer combinations were used for scoring of segregation in72 recombinant inbred lines obtained from a cross between C. arietinum and C. reticulation. Out of a total of 70 segregating AFLP markers, 58 were integrated in as existing linkage map mainly based on locus specific microsatellite and inter-simple sequence repeat markers, and two Fusarium resi¬stance genes. Although some clustering of AFLPs occurred, these markers were distributed all over the nine linkage groups of the map. Therefore, AFLP markers revealed to be useful for the satura¬tion of the existing chickpea map.
Development of AFLP markers for mapping in chickpea
Sonnante G;
1998
Abstract
In the AFLP technique has been proposed as a valuable tool for genetic mapping in plant species. In the present contribution, the application of AFLP was investigated for the generation of markers useful for genetic mapping in chickpea. A total of 48 primer combinations were tested in Cicer arietinum ICC 4958, susceptible to the pathogen Fusarium oxysporum f.sp. ciceri and in C. reticu-latum PI 489777, resistant to the same fungus. The electrophoretic pattern showed an average of 38 bands for each primer combination and the polymorphic fragments between the two species were comprised between 1 and 15 fragments, Twelve primer combinations did not provide interpretable results. The six most polymorphic primer combinations were used for scoring of segregation in72 recombinant inbred lines obtained from a cross between C. arietinum and C. reticulation. Out of a total of 70 segregating AFLP markers, 58 were integrated in as existing linkage map mainly based on locus specific microsatellite and inter-simple sequence repeat markers, and two Fusarium resi¬stance genes. Although some clustering of AFLPs occurred, these markers were distributed all over the nine linkage groups of the map. Therefore, AFLP markers revealed to be useful for the satura¬tion of the existing chickpea map.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
