Artichoke (Cynara cardunculus L. var. scolymus (L.) Fiori) is an outcrossing species, which is usually propagated vegetatively. A wide morphological variation is present in the artichoke germplasm of the world collection held at the Germplasm Istitute, Bari, Italy. Some varieties or ecotypes have a limited geographic distribution and often take their name from the area where they are cultivated. In some cases, the same variety can be called with different names in different regions, causing synonymy. Moreover, the varietal belonging is made mainly according to the head morphology, size and colour and this is only possible when the plant reaches its reproductive stage. Therefore, when in autumn the green shoots are collected from the mother plant for propagation, it is not possible to determine the exact variety. This is also of major importance for plant collectors and genebanks that aim to eliminate duplicates from their collections. In order to evaluate the potential of several molecular markers in distinguishing among artichoke varieties and wild relatives, also for the possible valorization of underutilized germplasm, a RAPD analysis was started on 32 cultivated and two wild (Cynara cardunculus var. cardunculus L.) samples. Arbitrary decamer primers were used to amplify genomic DNA and 18 of them produced 69 interpretable polymorphic fragments. An intra-accession analysis using 4 polymorphic primers revealed that no RAPD variation was detected between individuals, on the other hand, the combination of polymorphic fragments allowed to distinguish all the material analysed, except for two types which had an identical banding pattern. Major differences were observed between cultivated and wild samples.
Characterization of artichoke genotypes using RAPD markers
Sonnante G;De Paolis A;Perrino P
2000
Abstract
Artichoke (Cynara cardunculus L. var. scolymus (L.) Fiori) is an outcrossing species, which is usually propagated vegetatively. A wide morphological variation is present in the artichoke germplasm of the world collection held at the Germplasm Istitute, Bari, Italy. Some varieties or ecotypes have a limited geographic distribution and often take their name from the area where they are cultivated. In some cases, the same variety can be called with different names in different regions, causing synonymy. Moreover, the varietal belonging is made mainly according to the head morphology, size and colour and this is only possible when the plant reaches its reproductive stage. Therefore, when in autumn the green shoots are collected from the mother plant for propagation, it is not possible to determine the exact variety. This is also of major importance for plant collectors and genebanks that aim to eliminate duplicates from their collections. In order to evaluate the potential of several molecular markers in distinguishing among artichoke varieties and wild relatives, also for the possible valorization of underutilized germplasm, a RAPD analysis was started on 32 cultivated and two wild (Cynara cardunculus var. cardunculus L.) samples. Arbitrary decamer primers were used to amplify genomic DNA and 18 of them produced 69 interpretable polymorphic fragments. An intra-accession analysis using 4 polymorphic primers revealed that no RAPD variation was detected between individuals, on the other hand, the combination of polymorphic fragments allowed to distinguish all the material analysed, except for two types which had an identical banding pattern. Major differences were observed between cultivated and wild samples.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
