Effects of sulphurous gases in two CO2 springs on total sulphur and thiols in acorns and oak seedlings

Quercus ilex and Quercus pubescens acorns were collected at two CO2 springs in Tuscany (Bossoleto and Viterbo, resp.). In addition to elevated CO2, these springs are a source of H2S and SO2. Acorns from a comparable field site distant from CO2 springs were used as controls. Acorns from CO2 springs contained significantly higher sulphur concentrations than controls (0.67 vs. 0.47 mgg1 dw in Q. ilex cotyledons and 1.01 vs. 0.80 in Q. pubescens) and, in Q. ilex, also more glutathione (1634 vs. 1393 nmol g1 dw). When acorns were germinated and seedlings grown under ambient air conditions in the Botanical Garden in Graz for one year, leaves of Q. pubescens grown from CO2 spring acorns showed significantly higher S-concentrations than leaves grown from control acorns (2.27 mgg1 vs. 1.56mg g1 dw), and those of Q. ilex higher glutathione concentrations (929 vs. 462 nmol g1 dw, CO2 spring vs. control acorns). One-year-old Q. ilex seedlings grown from CO2 spring acorns also showed elevated rates of chromosomal aberrations in root tips (a widely used test for environmental impacts), suggesting a permanent stress situation of the seedlings originating from the CO2 springs.