Despite the advent of high-output, recombinant DNA-based screening strategies, many important protein-protein interactions in the plant cell have been and still are revealed using co-sedimentation, affinity chromatography and other affinity techniques, co-immunoprecipitation and cross-linking. The advantages of these techniques, the care that should be taken interpreting the data obtained and the possible ways to overcome pitfalls are illustrated.
Physical methods
Vitale A
2002
Abstract
Despite the advent of high-output, recombinant DNA-based screening strategies, many important protein-protein interactions in the plant cell have been and still are revealed using co-sedimentation, affinity chromatography and other affinity techniques, co-immunoprecipitation and cross-linking. The advantages of these techniques, the care that should be taken interpreting the data obtained and the possible ways to overcome pitfalls are illustrated.File in questo prodotto:
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