Mercury as one of heavy metals belongs among the oldest and the best-known toxicants and contaminations of environment. In this study the effects of mercuric chloride on the motility and structural integrity of rabbit spermatozoa in vitro were investigated. The spermatozoa motility was evaluated using CASA method and Annexin analysis was used for detection of structural changes. The concentration of mercury in medium vary from 5.0 to 83.3 ¼g HgCl2/mL. At Time 0 the highest motility in control group was detected (67.09±8.72%). Motility in groups with mercury administration was decreased in comparison with control. Significant differences were detected in groups with 50.0 83.3 ¼g HgCl2/mL (p<0.001). After 60 and 120 minutes of incubation with mercury chloride the motility was significantly decreased in almost all experimental groups. Progressive motility had a decreasing trend in all experimental groups. Significant differences in group 6.25 83.3 ¼g HgCl2/mL at Time 60 and 120 were found. Significant differences were detected in all experimental groups, expect the group with lowest mercury chloride concentration. The concentration decreasing spermatozoa progressive motility to 50% of control was detected for groups 50.0 83.3 at Time 0, for groups 12.5 83.3 at Time 60 and 120, decreasing from 36.46±18.73% to 1.03±2.50%. Detail evaluation of spermatozoa distance (DAP, DCL, and DSL) and velocity (VAP, VCL, and VSL) parameters as well as straightness (STR), linearity (LIN), wobble (WOB), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF) of spermatozoa were decreased in groups with the highest mercury concentration in comparison with control group in all time periods. Detection of spermatozoa with disordered membrane using Annexin analysis was implemented for groups with higher mercury concentration and control. Analysis proved higher occurrence of positive spermatozoa in the mercury exposed groups. Few Annexin positive reactions from all spermatozoa were detected in control group. In mercury exposed groups positive reaction proved alteration in anterial part of head (acrosome), connection part (connection piece) and in mitochondrial segment. Detected data evidently confirm adverse effects of high mercury chloride concentrations in rabbit semen on spermatozoa motility parameters.
In vitro toxicity of mercuric chloride on rabbit spermatozoa motility and cell membrane integrity
Flavia Pizzi;
2010
Abstract
Mercury as one of heavy metals belongs among the oldest and the best-known toxicants and contaminations of environment. In this study the effects of mercuric chloride on the motility and structural integrity of rabbit spermatozoa in vitro were investigated. The spermatozoa motility was evaluated using CASA method and Annexin analysis was used for detection of structural changes. The concentration of mercury in medium vary from 5.0 to 83.3 ¼g HgCl2/mL. At Time 0 the highest motility in control group was detected (67.09±8.72%). Motility in groups with mercury administration was decreased in comparison with control. Significant differences were detected in groups with 50.0 83.3 ¼g HgCl2/mL (p<0.001). After 60 and 120 minutes of incubation with mercury chloride the motility was significantly decreased in almost all experimental groups. Progressive motility had a decreasing trend in all experimental groups. Significant differences in group 6.25 83.3 ¼g HgCl2/mL at Time 60 and 120 were found. Significant differences were detected in all experimental groups, expect the group with lowest mercury chloride concentration. The concentration decreasing spermatozoa progressive motility to 50% of control was detected for groups 50.0 83.3 at Time 0, for groups 12.5 83.3 at Time 60 and 120, decreasing from 36.46±18.73% to 1.03±2.50%. Detail evaluation of spermatozoa distance (DAP, DCL, and DSL) and velocity (VAP, VCL, and VSL) parameters as well as straightness (STR), linearity (LIN), wobble (WOB), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF) of spermatozoa were decreased in groups with the highest mercury concentration in comparison with control group in all time periods. Detection of spermatozoa with disordered membrane using Annexin analysis was implemented for groups with higher mercury concentration and control. Analysis proved higher occurrence of positive spermatozoa in the mercury exposed groups. Few Annexin positive reactions from all spermatozoa were detected in control group. In mercury exposed groups positive reaction proved alteration in anterial part of head (acrosome), connection part (connection piece) and in mitochondrial segment. Detected data evidently confirm adverse effects of high mercury chloride concentrations in rabbit semen on spermatozoa motility parameters.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
