The present paper deals with the analysis of natural polymorphism in a selected AOX gene of the medicinal plant St. Johns Wort. Four partial AOX gene sequences were isolated from the genomic DNA of a wild plant of Hypericum perforatum L.. Three genes belong to the sub-family AOX1 (HpAOX1a, b and c) and one to the sub-family AOX2 (HpAOX2). The partial sequence of HpAOX1b demonstrated PCR-fragment size variation due to variable lengths in two introns. EPIC-PCR displayed the same two-band pattern in six plants from a collection. Both fragments showed identical sequences for all exons. However, each of the two introns demonstrated an InDel, in identical positions for all plants, that counted for the difference in the two fragment sizes. The InDel in intron1 influenced the predictability of a pre-microRNA site. The almost identical PCR-fragment pattern was characterized by a high variability in the sequences. The InDels in both introns were linked to repetitive SNPs. The polymorphic pattern obtained by InDels and SNPs from both fragments together was appropriate to discriminate between all individual plants. We suggest that AOX sequence polymorphism in H. perforatum can be used for studies on gene diversity and biodiversity. Further, we conclude that AOX sequence polymorphism of individual plants should be considered in biological studies on AOX activity to exclude the influence of genetic diversity. The identified polymorphic fragments are available to be explored in future experiments as a source for functional marker development related to the characterization of origins/accessions and agronomic traits, such as plant growth, development and yield stability.
Intron polymorphism pattern in AOX1b of wild St John s wort (Hipericum perforatum) allows discrimination between individual plants.
Breviario D;
2009
Abstract
The present paper deals with the analysis of natural polymorphism in a selected AOX gene of the medicinal plant St. Johns Wort. Four partial AOX gene sequences were isolated from the genomic DNA of a wild plant of Hypericum perforatum L.. Three genes belong to the sub-family AOX1 (HpAOX1a, b and c) and one to the sub-family AOX2 (HpAOX2). The partial sequence of HpAOX1b demonstrated PCR-fragment size variation due to variable lengths in two introns. EPIC-PCR displayed the same two-band pattern in six plants from a collection. Both fragments showed identical sequences for all exons. However, each of the two introns demonstrated an InDel, in identical positions for all plants, that counted for the difference in the two fragment sizes. The InDel in intron1 influenced the predictability of a pre-microRNA site. The almost identical PCR-fragment pattern was characterized by a high variability in the sequences. The InDels in both introns were linked to repetitive SNPs. The polymorphic pattern obtained by InDels and SNPs from both fragments together was appropriate to discriminate between all individual plants. We suggest that AOX sequence polymorphism in H. perforatum can be used for studies on gene diversity and biodiversity. Further, we conclude that AOX sequence polymorphism of individual plants should be considered in biological studies on AOX activity to exclude the influence of genetic diversity. The identified polymorphic fragments are available to be explored in future experiments as a source for functional marker development related to the characterization of origins/accessions and agronomic traits, such as plant growth, development and yield stability.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
