A thermostable thioredoxin reductase isolated from S. solfataricus (SsTrxR) was successfully crystd. in the absence and in the presence of NADP. Two different crystal forms were obtained. Crystals of the form that yielded higher resoln. data (1.8 .ANG.) belonged to space group P212121, with unit-cell parameters a = 76.77, b = 120.68, and c = 126.85 .ANG.. The structure of the enzyme was solved by MAD methods using the anomalous signal from the Se atoms of selenomethionine-labeled SsTrxR.
Crystallization and preliminary x-ray crystallographic analysis of Sulfolobus solfataricus thioredoxin reductase
Ruggiero Alessia;Vitagliano Luigi
2005
Abstract
A thermostable thioredoxin reductase isolated from S. solfataricus (SsTrxR) was successfully crystd. in the absence and in the presence of NADP. Two different crystal forms were obtained. Crystals of the form that yielded higher resoln. data (1.8 .ANG.) belonged to space group P212121, with unit-cell parameters a = 76.77, b = 120.68, and c = 126.85 .ANG.. The structure of the enzyme was solved by MAD methods using the anomalous signal from the Se atoms of selenomethionine-labeled SsTrxR.File in questo prodotto:
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