Gamma-aminobutyric acid (GABA) activated chloride currents were studied in rat cerebellum granule cells in culture by the whole cell patch-clamp technique. Both the peak and steady state currents were inhibited by 100 microM lanthanum. In the first case, inhibition is due to an increase of the EC50 for GABA. The inhibitory effect of lanthanum on the peak current at 3 microM GABA increased with the cation concentration. A tendency towards the same behavior was found also for the inhibition of the steady state current, at 3 microM GABA, as a function of lanthanum concentration, although inhibition in this case was lower. The comparison of the results with published data about the effects of lanthanum on recombinant GABA(A) receptors likely to occur in granule cells allows suggestions about the receptor types giving, respectively, the peak and the steady state component.
Modulation by lanthanum ions of gamma-aminobutyric acid(A) receptors of rat cerebellum granule cells in culture: clues on their subunit composition.
2001
Abstract
Gamma-aminobutyric acid (GABA) activated chloride currents were studied in rat cerebellum granule cells in culture by the whole cell patch-clamp technique. Both the peak and steady state currents were inhibited by 100 microM lanthanum. In the first case, inhibition is due to an increase of the EC50 for GABA. The inhibitory effect of lanthanum on the peak current at 3 microM GABA increased with the cation concentration. A tendency towards the same behavior was found also for the inhibition of the steady state current, at 3 microM GABA, as a function of lanthanum concentration, although inhibition in this case was lower. The comparison of the results with published data about the effects of lanthanum on recombinant GABA(A) receptors likely to occur in granule cells allows suggestions about the receptor types giving, respectively, the peak and the steady state component.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
