Acetyl esterase (acetic-ester acetylhydrolase, EC 3.1.1.6) from citrus peel, whose natural role is not well known, catalyses, in vitro, the hydrolysis of acetyl groups from a wide range of substrates. This enzyme was extracted from Mediterranean orange peel, largely available in Italy, and purified 190-fold by a single chromatographic step on Sepabeads FP-HG. SDS polyacrylamide gel electrophoresis of the purified enzyme showed a major protein band, corresponding to a molecular mass of 45 kDa. Both free and immobilised enzyme were used in biotransformations. The enzyme removed the acetyl group in the 3 position of ?-lactamic antibiotics, such as cephalosporin C and the intermediate 7-aminocephalosporanic acid with >=98% conversion and 91-93% product yield. © 2004 Taylor & Francis Ltd.
Acetyl esterase from mediterranean oranges: partial purification, immobilisation and biotransformations
Pasta P;Zambianchi F;
2004
Abstract
Acetyl esterase (acetic-ester acetylhydrolase, EC 3.1.1.6) from citrus peel, whose natural role is not well known, catalyses, in vitro, the hydrolysis of acetyl groups from a wide range of substrates. This enzyme was extracted from Mediterranean orange peel, largely available in Italy, and purified 190-fold by a single chromatographic step on Sepabeads FP-HG. SDS polyacrylamide gel electrophoresis of the purified enzyme showed a major protein band, corresponding to a molecular mass of 45 kDa. Both free and immobilised enzyme were used in biotransformations. The enzyme removed the acetyl group in the 3 position of ?-lactamic antibiotics, such as cephalosporin C and the intermediate 7-aminocephalosporanic acid with >=98% conversion and 91-93% product yield. © 2004 Taylor & Francis Ltd.File | Dimensione | Formato | |
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