A novel polysaccharide was isolated from the extremophile Gram-negative bacterium Thermus thermophilus HB8. Its structural characterization employed compositional and methylation analyses, and 2D 1H and 13C NMR spectroscopy. Various chemical degradations (methanolysis and Smith degradation) were also carried out in order to depolymerise this complex macromolecule, and subsequent chemical and spectroscopic analyses were also carried out on the obtained fragments. All the data suggested a novel polymeric structure that consists of a disaccharide repeating unit to which a branching trisaccharide chain is linked, although not stoichiometrically.
Structural analysis of a novel polysaccharide of the lipopolysaccharide-deficient extremophile Gram-negative bacterium Thermus thermophilus HB8.
I Romano;B Nicolaus;
2004
Abstract
A novel polysaccharide was isolated from the extremophile Gram-negative bacterium Thermus thermophilus HB8. Its structural characterization employed compositional and methylation analyses, and 2D 1H and 13C NMR spectroscopy. Various chemical degradations (methanolysis and Smith degradation) were also carried out in order to depolymerise this complex macromolecule, and subsequent chemical and spectroscopic analyses were also carried out on the obtained fragments. All the data suggested a novel polymeric structure that consists of a disaccharide repeating unit to which a branching trisaccharide chain is linked, although not stoichiometrically.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
