We report here a 31P nuclear magnetic resonance (31P-NMR) investigation of buffaloes milk, milk ultrafiltrate, and phospholipids from milk fat; corresponding data were also acquired for cows samples. In milk samples, we identified glycerophosphorylcoline, glycerophosphorylethanolamine and inorganic orthophosphate resonances, together with a broad peak assigned to serylphosphate residues of casein. Ultrafiltrate samples of buffaloes milk showed the presence of several phosphorous signals and 10 of them (inorganic phosphate, phosphocreatine, glycerophosphorylcoline, glycerophosphorylethanolamine, N-acetylglucosamine-1-phosphate, glucose-6-phosphate, galactose-1-phosphate, phosphorylethanolamine, phosphorylcoline, and glycerol-1-phosphate) were unambiguously identified by addition of pure standards. In phospholipids fractions from buffaloes and cows milks, we clearly identified phosphatidylcholine, phosphatidylethanolamine, sphingomyelin, and phosphatidylinositol. By comparing 31P-NMR data from buffaloes and cows milks, we concluded that the amount (% mol) of phosphorylated compounds correspond in both milks.
Characterization of buffalo milk by 31P-nuclear magnetic resonance spectroscopy
Andreotti G;MOTTA A
2006
Abstract
We report here a 31P nuclear magnetic resonance (31P-NMR) investigation of buffaloes milk, milk ultrafiltrate, and phospholipids from milk fat; corresponding data were also acquired for cows samples. In milk samples, we identified glycerophosphorylcoline, glycerophosphorylethanolamine and inorganic orthophosphate resonances, together with a broad peak assigned to serylphosphate residues of casein. Ultrafiltrate samples of buffaloes milk showed the presence of several phosphorous signals and 10 of them (inorganic phosphate, phosphocreatine, glycerophosphorylcoline, glycerophosphorylethanolamine, N-acetylglucosamine-1-phosphate, glucose-6-phosphate, galactose-1-phosphate, phosphorylethanolamine, phosphorylcoline, and glycerol-1-phosphate) were unambiguously identified by addition of pure standards. In phospholipids fractions from buffaloes and cows milks, we clearly identified phosphatidylcholine, phosphatidylethanolamine, sphingomyelin, and phosphatidylinositol. By comparing 31P-NMR data from buffaloes and cows milks, we concluded that the amount (% mol) of phosphorylated compounds correspond in both milks.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.