The aim of the present paper is to investigate intestinal absorption and toxicity of Fumonisin B1 (FB1) and its partially (PHFB1 and PHFB2) ant totally hydrolyzed (HFB1) metabolites, using the human intestinal cell line Caco-2, a very well known in vitro model of intestinal epithelium for absorption and metabolism studies. caco-2 cells were treated for 48 h with several toxin concentrations (in the range of 1-138 uM). At the end of exposure period, no significant variation on cell viability has been observed with all chemicals tested, either in undifferentiated cell or in differentiated ones, suggesting a poor toxicity of these mycotoxins for intestinal cells. In any case, FB1 appears the most active in this respect. For which concerns the cellular absorption, FB1,PHFB1 and PHFB2 arenever detected into Caco-2 cells. on thecontrary, a dose dependent absorption of HFB1 has been observed in differentiated cells, which express enzymatic and metabolic characteristics of mature enterocytes. Thus HFB1, losin the tricarballylic acid chain, is more bioavailable than FB1 on intestinal cells, supporting the hypothesis that in risk evaluation of fumonisin exposure its metabolites are also relevant.
Evaluation of Fumonisin B1 and its metabolites absorption and toxicity on intestinal cells line Caco-2.
2002
Abstract
The aim of the present paper is to investigate intestinal absorption and toxicity of Fumonisin B1 (FB1) and its partially (PHFB1 and PHFB2) ant totally hydrolyzed (HFB1) metabolites, using the human intestinal cell line Caco-2, a very well known in vitro model of intestinal epithelium for absorption and metabolism studies. caco-2 cells were treated for 48 h with several toxin concentrations (in the range of 1-138 uM). At the end of exposure period, no significant variation on cell viability has been observed with all chemicals tested, either in undifferentiated cell or in differentiated ones, suggesting a poor toxicity of these mycotoxins for intestinal cells. In any case, FB1 appears the most active in this respect. For which concerns the cellular absorption, FB1,PHFB1 and PHFB2 arenever detected into Caco-2 cells. on thecontrary, a dose dependent absorption of HFB1 has been observed in differentiated cells, which express enzymatic and metabolic characteristics of mature enterocytes. Thus HFB1, losin the tricarballylic acid chain, is more bioavailable than FB1 on intestinal cells, supporting the hypothesis that in risk evaluation of fumonisin exposure its metabolites are also relevant.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
