The effects of ionic liquid media on the activity of Candida antarctica lipase B in a simple transesterification reaction were studied. In ionic liquids containing dicyanimide, alkylsulfate, nitrate and lactate anions, which dissolved CaLB, the reaction was at least ten times slower than in [BMIm][BF4]. Only [Et3MeN][MeSO4] was an exception, as dissolved CaLB maintained its activity in this solvent. A cross-linked enzyme aggregate of CaLB was twice as active in [BMIm][dca] as in tert-butyl alcohol, whereas the free enzyme was irreversibly deactivated in this latter ionic liquid.

Structure and activity of Candida antarctica lipase B in ionic liquids

Francesco Secundo;
2006

Abstract

The effects of ionic liquid media on the activity of Candida antarctica lipase B in a simple transesterification reaction were studied. In ionic liquids containing dicyanimide, alkylsulfate, nitrate and lactate anions, which dissolved CaLB, the reaction was at least ten times slower than in [BMIm][BF4]. Only [Et3MeN][MeSO4] was an exception, as dissolved CaLB maintained its activity in this solvent. A cross-linked enzyme aggregate of CaLB was twice as active in [BMIm][dca] as in tert-butyl alcohol, whereas the free enzyme was irreversibly deactivated in this latter ionic liquid.
2006
Istituto di Chimica del Riconoscimento Molecolare - ICRM - Sede Milano
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.14243/167441
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