Vein necrosis (VN), a virus-like disease of the grapevine first identified in France by Legin and Vuittenez (1973), induces necrosis of the veinlets on the underside of the leaf blade of its specific indicator Vitis rupestris x Vitis berlandieri 110 R. The disease is latent in all European grapevine cultivars and in most American rootstock species and hybrids, has a worldwide distribution, and has no known agent. VN is very common in southern Italy, so that over 50% (87 of 173) of the grapevine accessions selected in the course of sanitary improvement programmes in the last few years indexed positive on 110R. As assessed by ELISA, the same vines had a very low rate of infection (<3%) by detrimental viruses (GFLV, GVA, GVB, GLRaV-1, GLRaV-2, GLRaV-3, GFkV) commonly tested for in the course of selection. When the VN-positive 110R indicators were checked by PCR and Western blot for the presence of Grapevine rupestris stem pitting-associated virus (GRSPaV) a strikingly high association (over 95%) was observed between this virus and VN symptoms. Likewise, the totality of 72 mother plants of Vitis rupestris used as indicators in indexing trials and recently discovered to be infected by GRSPaV, induced VN reactions following grafting onto 110 R, whilst no VN reactions developed in 110R vines top-grafted on a single GRSPaV-free V. rupestris. Moreover, symptomatic bottom leaves from VN-infected 110R vines were consistently positive for GRSPaV whereas the symptomless top leaves from the same shoots were not. The very close association between GRSPaV and VN symptoms strongly suggests that this virus may be implicated in the aetiology of VN.
Is grapevine vein necrosis a reaction to Grapevine rupestris stem pitting-associated virus ?
Boscia D;Savino V;La Notte P;Minafra A;
2004
Abstract
Vein necrosis (VN), a virus-like disease of the grapevine first identified in France by Legin and Vuittenez (1973), induces necrosis of the veinlets on the underside of the leaf blade of its specific indicator Vitis rupestris x Vitis berlandieri 110 R. The disease is latent in all European grapevine cultivars and in most American rootstock species and hybrids, has a worldwide distribution, and has no known agent. VN is very common in southern Italy, so that over 50% (87 of 173) of the grapevine accessions selected in the course of sanitary improvement programmes in the last few years indexed positive on 110R. As assessed by ELISA, the same vines had a very low rate of infection (<3%) by detrimental viruses (GFLV, GVA, GVB, GLRaV-1, GLRaV-2, GLRaV-3, GFkV) commonly tested for in the course of selection. When the VN-positive 110R indicators were checked by PCR and Western blot for the presence of Grapevine rupestris stem pitting-associated virus (GRSPaV) a strikingly high association (over 95%) was observed between this virus and VN symptoms. Likewise, the totality of 72 mother plants of Vitis rupestris used as indicators in indexing trials and recently discovered to be infected by GRSPaV, induced VN reactions following grafting onto 110 R, whilst no VN reactions developed in 110R vines top-grafted on a single GRSPaV-free V. rupestris. Moreover, symptomatic bottom leaves from VN-infected 110R vines were consistently positive for GRSPaV whereas the symptomless top leaves from the same shoots were not. The very close association between GRSPaV and VN symptoms strongly suggests that this virus may be implicated in the aetiology of VN.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
