This study aimed to determine whether modification of physiological parameters could be detected remotely by monitoring the spectral reflectance of olive leaves in response to different degrees of drought. Three different drought intensities were simulated: (a) a mild drought by feeding abscisic acid to detached branches; (b) a rapid and severe drought by detaching leaves and letting them dry over several hours; (c) a relatively slow drought caused by withholding water to potted olive plants. The three degrees of stress affected gas exchange and chlorophyll fluorescence. When the inhibition of photosynthesis occurred within an hour it was not accompanied by a parallel reduction in chlorophyll concentration in the carotenoid to chlorophyll ratio. Consequently, changes in spectral reflectance in the visible region, e.g. in PRI (photochemical reflectance index) and FRI (fluorescence reflectance indices) were not significantly induced. In contrast, when the inhibition of photosynthesis caused by slow developing drought was prolonged (i.e. more than 24 hours) and led to a decrease in chlorophyll concentration and to a simultaneous increase in carotenoid to chlorophyll ratio, there were significant changes in the visible region of the leaf spectral reflectance and, in turn, in PRI and FRI. We defined 16 new reference wavelengths, from visible to SWIR regions, which are sensitive to both fast-developing and slow-developing stresses. These reference wavelengths were used to develop an algorithm, the Relative Reflectance Increment (RRI), that was linearly related to changes in relative water content (RWC, r(2)=0.733). This algorithm showed that the 1455 nm wavelength is highly affected by drought. This wavelength was therefore used to elaborate the water content reflectance index that was inversely related to RWC (r(2)=0.702).
Associated changes in physiological parameters and spectral reflectance indices in olive (Olea europaea L.) leaves in response to different levels of water stress.
F Loreto;M Centritto
2008
Abstract
This study aimed to determine whether modification of physiological parameters could be detected remotely by monitoring the spectral reflectance of olive leaves in response to different degrees of drought. Three different drought intensities were simulated: (a) a mild drought by feeding abscisic acid to detached branches; (b) a rapid and severe drought by detaching leaves and letting them dry over several hours; (c) a relatively slow drought caused by withholding water to potted olive plants. The three degrees of stress affected gas exchange and chlorophyll fluorescence. When the inhibition of photosynthesis occurred within an hour it was not accompanied by a parallel reduction in chlorophyll concentration in the carotenoid to chlorophyll ratio. Consequently, changes in spectral reflectance in the visible region, e.g. in PRI (photochemical reflectance index) and FRI (fluorescence reflectance indices) were not significantly induced. In contrast, when the inhibition of photosynthesis caused by slow developing drought was prolonged (i.e. more than 24 hours) and led to a decrease in chlorophyll concentration and to a simultaneous increase in carotenoid to chlorophyll ratio, there were significant changes in the visible region of the leaf spectral reflectance and, in turn, in PRI and FRI. We defined 16 new reference wavelengths, from visible to SWIR regions, which are sensitive to both fast-developing and slow-developing stresses. These reference wavelengths were used to develop an algorithm, the Relative Reflectance Increment (RRI), that was linearly related to changes in relative water content (RWC, r(2)=0.733). This algorithm showed that the 1455 nm wavelength is highly affected by drought. This wavelength was therefore used to elaborate the water content reflectance index that was inversely related to RWC (r(2)=0.702).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.