Novel functional materials based on the idea of engineering the interfaces and the interactions of the organic molecule with inorganic surfaces at the nanoscale can give a strong impulse to biomedical application for protein or aptamer detection. A lot of efforts are devoted to the realization of lab-on-a-chip, focusing on the combination of nano and micro technologies to deliver compact, reliable diagnostic tools, with improved analysis performances. Devices based on optical response using integrated waveguides has been recently considered an attractive sensing modality for protein recognition, but the functionalization of materials like silicon nitride and oxides, typically used in waveguides is still a challenge. We investigated the interaction of a small molecule with the two technological surfaces, a stoichiometric Si3N4 growth by LPCVD apparatus and SiO2. We have functionalized the surfaces in vacuum by using a Supersonic Molecular Beam seeded by naphtalene dicarboxilic acid, the so called SuMBD deposition approach. The kinetics properties of the beam have been investigated by using a Time of Flight apparatus, estimating a kinetic energy of the molecule in a supersonic beam of 6.5 eV. The degree of surface site functionalization has been evaluated by optical luminescence of an amino fluorescein bonded with the naphthalene molecule at the surface. The optical properties and the molecule distribution on the surfaces has been analyzed also by a FT-IR measurements. We studied the growth mechanism and the chemical/physical properties of the interface organic/inorganic with in-situ surface electron spectroscopies (UPS, XPS). The good results in terms of molecular packing density on the inorganic surfaces prove the efficacy and reliability of the SuMBD approach in functionalizing different materials, that typically requires the use of different wet chemical approaches that are not always compatible with in vacuum MBE growth techniques of nano and micro technologies at the basis of these lab-on-a-chip devices.
Si3N4 and SiO2 functionalized surfaces with a naphthalene carboxylic acid by SuMBD for biosensing
Nardi Marco Vittorio;Verucchi Roberto;Aversa Lucrezia;Iannotta Salvatore
2011
Abstract
Novel functional materials based on the idea of engineering the interfaces and the interactions of the organic molecule with inorganic surfaces at the nanoscale can give a strong impulse to biomedical application for protein or aptamer detection. A lot of efforts are devoted to the realization of lab-on-a-chip, focusing on the combination of nano and micro technologies to deliver compact, reliable diagnostic tools, with improved analysis performances. Devices based on optical response using integrated waveguides has been recently considered an attractive sensing modality for protein recognition, but the functionalization of materials like silicon nitride and oxides, typically used in waveguides is still a challenge. We investigated the interaction of a small molecule with the two technological surfaces, a stoichiometric Si3N4 growth by LPCVD apparatus and SiO2. We have functionalized the surfaces in vacuum by using a Supersonic Molecular Beam seeded by naphtalene dicarboxilic acid, the so called SuMBD deposition approach. The kinetics properties of the beam have been investigated by using a Time of Flight apparatus, estimating a kinetic energy of the molecule in a supersonic beam of 6.5 eV. The degree of surface site functionalization has been evaluated by optical luminescence of an amino fluorescein bonded with the naphthalene molecule at the surface. The optical properties and the molecule distribution on the surfaces has been analyzed also by a FT-IR measurements. We studied the growth mechanism and the chemical/physical properties of the interface organic/inorganic with in-situ surface electron spectroscopies (UPS, XPS). The good results in terms of molecular packing density on the inorganic surfaces prove the efficacy and reliability of the SuMBD approach in functionalizing different materials, that typically requires the use of different wet chemical approaches that are not always compatible with in vacuum MBE growth techniques of nano and micro technologies at the basis of these lab-on-a-chip devices.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
