We report on a compact twin-beam interferometer that can be adopted as a flexible diagnostic tool in microfluidic platforms with twofold functionality. The novel configuration allows 3D tracking of micro-particles and, at same time, can simultaneously furnish Quantitative Phase-contrast maps of tracked micro-objects by interference microscopy, without changing the configuration. Experimental demonstration is given on for in vitro cells in a microfluidic environment. (C) 2011 Optical Society of America
Twin-beams digital holography for 3D tracking and quantitative phase-contrast microscopy in microfluidics
Memmolo Pasquale;Finizio Andrea;Paturzo Melania;Miccio Lisa;Ferraro Pietro
2011
Abstract
We report on a compact twin-beam interferometer that can be adopted as a flexible diagnostic tool in microfluidic platforms with twofold functionality. The novel configuration allows 3D tracking of micro-particles and, at same time, can simultaneously furnish Quantitative Phase-contrast maps of tracked micro-objects by interference microscopy, without changing the configuration. Experimental demonstration is given on for in vitro cells in a microfluidic environment. (C) 2011 Optical Society of AmericaFile in questo prodotto:
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