Several malignant cell lines are resistant to CD95(Apol/Fas)-mediated apoptosis, even when the CD95 receptor is highly expressed. Sensitivity to CD95-1nduced apoptosis can be restored using different molecules. In this study, we showed that quercetin, a naturally occuring flavonoid, in association with the agonistic anti-CD95 monoclonal antibody, increases DNA fragmentation and caspase-3 activity in HPB-ALL cells. These cells have been selected for their known resistance to CD95-induced apoptosis, At molecular level, quercetin lowers the level of intracellular reactive oxygen species, reduces mitochondrial transmembrane potential, thereby leaving the expression of CD95 receptor unchanged.
Quercetin and anti-CD95(Fas/Apo1) enhance apoptosis in HPB-ALL cell line.
Russo M;Palumbo R;Tedesco I;Russo P;Iacomino G;Russo GL
1999
Abstract
Several malignant cell lines are resistant to CD95(Apol/Fas)-mediated apoptosis, even when the CD95 receptor is highly expressed. Sensitivity to CD95-1nduced apoptosis can be restored using different molecules. In this study, we showed that quercetin, a naturally occuring flavonoid, in association with the agonistic anti-CD95 monoclonal antibody, increases DNA fragmentation and caspase-3 activity in HPB-ALL cells. These cells have been selected for their known resistance to CD95-induced apoptosis, At molecular level, quercetin lowers the level of intracellular reactive oxygen species, reduces mitochondrial transmembrane potential, thereby leaving the expression of CD95 receptor unchanged.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
