A capillary gas chromatographic method was developed to determine qualitatively and quantitatively the pentosan content in wood species of Italian forestry biomass. The proposed methodology aims at determining the composition of the single components of pectins and hemicelluloses, arabinans and xylans. Wood samples of Pinus nigricans Host. were prepared by acid hydrolysis with TFA (trifluoroacetic acid), evaporation by Rotavapor, dry over P2O5 and final silanization with Trisil (Pierce) before the gas chromatographic analysis. The principle of official method is the conversion of the pentosans to furfural by hydrochloric acid and it estimates the total amount of pentosans without a determination of the individual sugars. The proposed method is advantageous because is able to determine directly the pentosans and to discriminate between the individual sugar components
Capillary GLC method for determination of single pentosans of Pinus nigricans Host.
Franci M;Michelozzi;
1999
Abstract
A capillary gas chromatographic method was developed to determine qualitatively and quantitatively the pentosan content in wood species of Italian forestry biomass. The proposed methodology aims at determining the composition of the single components of pectins and hemicelluloses, arabinans and xylans. Wood samples of Pinus nigricans Host. were prepared by acid hydrolysis with TFA (trifluoroacetic acid), evaporation by Rotavapor, dry over P2O5 and final silanization with Trisil (Pierce) before the gas chromatographic analysis. The principle of official method is the conversion of the pentosans to furfural by hydrochloric acid and it estimates the total amount of pentosans without a determination of the individual sugars. The proposed method is advantageous because is able to determine directly the pentosans and to discriminate between the individual sugar componentsI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
