A virus was isolated in Italy from Tanlus communis in which it caused symptoms of red mosaic and leaf dstortion. The virus, for which the name tamus red mosaic virus (TRMV) is suggested, was differentiated from other potexviruses or possible potexviruses on the basis of host range. particle properties and serology. In crude preparations, TRMV had elongated particles of modal length 550-560 nm and diameter of 13 nm. TRMV was easily purified from Chenopodium quinoa and had a buoyant density of 1.34 g/cm3 in CsCl and 1.27 g/cm3 in Cs2S04. An antiserum to TRMV was obtained with a titre of 1 1023 in slide precipitin tests. TRMV was related to. but distinct from. tamus latent birus. a potexvirus isolated from Tamus commutlis in the UK, and more distantly related to potato virus X, cactus virus X and commelina virus X. Polyacrylamide gel electrophoresis (PAGE) indicated that the birus particles contained a single protein species of estimated molecular weight 25.2 kDa. Enzymatic digestions. agarose gel electrophoresis and infectivity of the isolated nucleic acid indicated that the virus has a single major molecule of single-stranded positive-sense RNA of estimated molecular weight 2120 kDa (ca. 6.4 kb).
Tamus red mosaic virus, a potexvirus from black bryony (Tamus communis).
CACIAGLI P;
1993
Abstract
A virus was isolated in Italy from Tanlus communis in which it caused symptoms of red mosaic and leaf dstortion. The virus, for which the name tamus red mosaic virus (TRMV) is suggested, was differentiated from other potexviruses or possible potexviruses on the basis of host range. particle properties and serology. In crude preparations, TRMV had elongated particles of modal length 550-560 nm and diameter of 13 nm. TRMV was easily purified from Chenopodium quinoa and had a buoyant density of 1.34 g/cm3 in CsCl and 1.27 g/cm3 in Cs2S04. An antiserum to TRMV was obtained with a titre of 1 1023 in slide precipitin tests. TRMV was related to. but distinct from. tamus latent birus. a potexvirus isolated from Tamus commutlis in the UK, and more distantly related to potato virus X, cactus virus X and commelina virus X. Polyacrylamide gel electrophoresis (PAGE) indicated that the birus particles contained a single protein species of estimated molecular weight 25.2 kDa. Enzymatic digestions. agarose gel electrophoresis and infectivity of the isolated nucleic acid indicated that the virus has a single major molecule of single-stranded positive-sense RNA of estimated molecular weight 2120 kDa (ca. 6.4 kb).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
