The major goals of our research have been the characterisation of juvenile and adult phases at the molecular level and the identification of genes either involved in phase transition or related to developmental specific traits. Peach was chosen as a model species since it features visible traits which are effective to distinguish and predict developmental phases. A set of cDNA markers was obtained by differential display experiments performed to compare mRNA expression in basal ( juvenile-like) and apical (adult vegetative) shoots derived from mature plants. Within these markers the RPS28 gene, coding for the S28 ribosomal protein, was found to be more expresswed in the leaves of basal shoots and its message was confined to areas involved in cell divisions. Though RPS28 was not found to be a strict specific marker, its splicing appeared to occur with lower efficiency with relation to leaf ageing. The mature RPS28 mRNA was most abundant in actively divinding tissues such as apical tips and forming leaves. Precursor accumulation in the co-presence of the mature product was found in fully expanded leaves and as the only product in the late stage tissue or senescent leaves. T characterise adult tissues the meristem fate related gene DNA mehtylase I was isolated and its expression investigated during the formation of vegetative and reproductive meristems which developed in co-axial buds. Distinct pattern of message localisation was observed in the two meristem types at very early developmental stages suggesting that the gene may be involved in regulating the competence of meristem layers which perceive the cell fate signals.
"Characterisation of juvenile and adult stages in woody species by means of DDRT-PCR markers and genes from heterologous species".
Giannino D;Mele G;Frugis G;Mariotti D
1999
Abstract
The major goals of our research have been the characterisation of juvenile and adult phases at the molecular level and the identification of genes either involved in phase transition or related to developmental specific traits. Peach was chosen as a model species since it features visible traits which are effective to distinguish and predict developmental phases. A set of cDNA markers was obtained by differential display experiments performed to compare mRNA expression in basal ( juvenile-like) and apical (adult vegetative) shoots derived from mature plants. Within these markers the RPS28 gene, coding for the S28 ribosomal protein, was found to be more expresswed in the leaves of basal shoots and its message was confined to areas involved in cell divisions. Though RPS28 was not found to be a strict specific marker, its splicing appeared to occur with lower efficiency with relation to leaf ageing. The mature RPS28 mRNA was most abundant in actively divinding tissues such as apical tips and forming leaves. Precursor accumulation in the co-presence of the mature product was found in fully expanded leaves and as the only product in the late stage tissue or senescent leaves. T characterise adult tissues the meristem fate related gene DNA mehtylase I was isolated and its expression investigated during the formation of vegetative and reproductive meristems which developed in co-axial buds. Distinct pattern of message localisation was observed in the two meristem types at very early developmental stages suggesting that the gene may be involved in regulating the competence of meristem layers which perceive the cell fate signals.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
