An isolate of barley yellow dwarf virus (BYDV) was recovered frorn dwarfed and yellowed winter barley (Hordeum vulgare L.) in northwest Italy by serial transmissions with Rhopalosiphum padi (L.) to Avena sativa L. As indicators of the process of selection, three parameters were used: the proportion of plants infected by R. padi and Sitobion avenae (Fabricius) in consecutive transmission tests; the reactions of the infected plants of each transmission test with the sera against RPVand MAV-like isolates; and the variability of the ELISA reactions within each group of plants tested. The isolate recovered was transmitted by R. padi and S. avenae with efficiencies of 92 and 11 %, respectively. It also reacted consistently with the sera against the MAV isolate (relatively high absorbance values) and the RPV isolate (relatively lower vaiues) in ELISA.
Recovery of an isolate of Barley Yellow Dwarf Virus from barley in Nortwest Italy.
Caciagli P
1990
Abstract
An isolate of barley yellow dwarf virus (BYDV) was recovered frorn dwarfed and yellowed winter barley (Hordeum vulgare L.) in northwest Italy by serial transmissions with Rhopalosiphum padi (L.) to Avena sativa L. As indicators of the process of selection, three parameters were used: the proportion of plants infected by R. padi and Sitobion avenae (Fabricius) in consecutive transmission tests; the reactions of the infected plants of each transmission test with the sera against RPVand MAV-like isolates; and the variability of the ELISA reactions within each group of plants tested. The isolate recovered was transmitted by R. padi and S. avenae with efficiencies of 92 and 11 %, respectively. It also reacted consistently with the sera against the MAV isolate (relatively high absorbance values) and the RPV isolate (relatively lower vaiues) in ELISA.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
