The disease first appeared in tomatoes under glass in southern Sardinia during Autumn 1988, and was very destructive, particularly in glasshouses where the whitefly Bemisia tabaci was found to be very numerous. The symptoms resembled those caused by tomato yellow leaf curl gerninivirus (TYLCV). The disease was transmitted to young tornato seedlings cv. Marmande, Rutgers and ltalpeel by grafting, and to the cv. Marmande also by B. tabaci, either collected from tomato plants on site or reared on graft-infected tomato plants. Symptoms, in plants kept at 25 CC with 18 h light, appeared about two weeks after graft or whitefly inoculation. DNAs were extracted from infected and healthy samples and probed in Southern blots with cloned TYLCV DNA. lnfected samples reacted specifically Nuclei of thin-sectioned cells from naturally and graft-infected tomato plants contained accumulations of geminivirus particles and fibrillar rings. Geminivirus particles were detected after negative staining with uranyl acetate in leaf dip preparations from naturally infected tomatoes and in partially purified virus preparations. The use of enzymes with cellulase and pectinase activity during virus extraction was found useful for purification. An antiserum is being prepared.
A geminivirus associated with a severe yellow leaf curl disease of tomato in Sardinia.
CACIAGLI P;ACCOTTO;G P;CONTI M;GALLITELLI D;G P;
1989
Abstract
The disease first appeared in tomatoes under glass in southern Sardinia during Autumn 1988, and was very destructive, particularly in glasshouses where the whitefly Bemisia tabaci was found to be very numerous. The symptoms resembled those caused by tomato yellow leaf curl gerninivirus (TYLCV). The disease was transmitted to young tornato seedlings cv. Marmande, Rutgers and ltalpeel by grafting, and to the cv. Marmande also by B. tabaci, either collected from tomato plants on site or reared on graft-infected tomato plants. Symptoms, in plants kept at 25 CC with 18 h light, appeared about two weeks after graft or whitefly inoculation. DNAs were extracted from infected and healthy samples and probed in Southern blots with cloned TYLCV DNA. lnfected samples reacted specifically Nuclei of thin-sectioned cells from naturally and graft-infected tomato plants contained accumulations of geminivirus particles and fibrillar rings. Geminivirus particles were detected after negative staining with uranyl acetate in leaf dip preparations from naturally infected tomatoes and in partially purified virus preparations. The use of enzymes with cellulase and pectinase activity during virus extraction was found useful for purification. An antiserum is being prepared.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
