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CHARACTERISATION OF THE PHENYLALANINE AMMONIA-LYASE GENES IN ARTICHOKE

DE PAOLIS A;D PIGNONE;G SONNANTE
2004
2004
Inglese
XLVIII Italian Society of Agricultural Genetics - SIFV-SIGA Joint Meeting
88-900622-5-8
Sì, ma tipo non specificato
15/18 Settembre, 2004
Lecce
The formation of free radicals is connected to the normal aerobic cell methabolism. The consumption of oxygen during the cell growth determines the formation of several oxygen free radicals, whose interaction with lipid molecules produces new free radicals: hydroperoxides and several peroxides. This group of radicals can interact with biological systems determining citotoxicity. It has been demonstrated that artichoke extracts are rich in phenolic compounds which protect cell membranes, show antibacterial, antioxidative, anti-HIV, bile expelling, hepatoprotective, urinative, and choleretic activities as well as the ability to inhibit cholesterol biosynthesis and LDL oxidation. The first enzymatic step in the pathway of phenilpropanoid biosynthesis is the deamination of phenylalanine by means of the enzyme phenylalanine ammonia-lyase (PAL). This enzyme has been characterised in several plant species and it is usually coded by more than one gene. In order to study the organisation of the gene/genes coding for PAL in artichoke, we have used primers designed on the basis of the information retrieved from the sequence databases. Primers were designed in two exons flanking the single intron included in the PAL gene. After amplification and gel separation, two DNA fragments were identified of about 550 and 1000 bp respectively. The two fragments were isolated and sequenced and revealed to share a high homology with the PAL genes from other Asteraceae, the family which the artichoke belongs to. The DNA fragments obtained are being used as a molecular probe to screen a genomic library in order to isolate the PAL gene/genes in artichoke and characterise its/their organisation. The isolation of mRNA from various tissues and the synthesis of cDNA will allow to follow the patterns of expression of PAL genes in artichoke.
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info:eu-repo/semantics/conferenceObject
none
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04 Contributo in convegno::04.02 Abstract in Atti di convegno
DE PAOLIS, A; Pignone, D; Sonnante, G
04.02 Abstract in Atti di convegno
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.14243/207864
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