Fluorescent In Situ Hybridization (FISH) technique, representsa versatile technique which combines the accuracy of molecularhybridization with fluorescence-labeling and cytogeneticdescription of chromosome morphology, to provide a physicallocalization of genes within DNA segments of interest. he actual resolution of FISHallows distinction of DNA sequences separated a few megabaseapart in metaphase or meiotic prophase chromosomes and a low-end resolution limit around 50 kilobase pairs in interphase nucleiin which, however, no banding pattern can be obtained.Oligonucleotide-PRimed IN Situ DNA synthesis (PRINS)technique has been shown be a rapid method, alternative toFluorescent In Situ Hybridization, which has been utilized forstudying the distribution of the alphoid sequences of the humanX chromosome .
LOCALIZATION OF SINGLE-COPY GENE BY PRINS TECHNIQUE
Cinti, Caterina
Writing – Review & Editing
;Santi, SpartacoMembro del Collaboration Group
1993
Abstract
Fluorescent In Situ Hybridization (FISH) technique, representsa versatile technique which combines the accuracy of molecularhybridization with fluorescence-labeling and cytogeneticdescription of chromosome morphology, to provide a physicallocalization of genes within DNA segments of interest. he actual resolution of FISHallows distinction of DNA sequences separated a few megabaseapart in metaphase or meiotic prophase chromosomes and a low-end resolution limit around 50 kilobase pairs in interphase nucleiin which, however, no banding pattern can be obtained.Oligonucleotide-PRimed IN Situ DNA synthesis (PRINS)technique has been shown be a rapid method, alternative toFluorescent In Situ Hybridization, which has been utilized forstudying the distribution of the alphoid sequences of the humanX chromosome .I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
