We have developed a new experimental approach, Temperature Derivative X-ray Absorption Spectroscopy (TDXAS), to measure the conformational landscape of a metalloprotein and how it depends on the selected conformational coordinate of the metal active site structure. We have recorded the temperature variation of a selected feature of the X ray Absorption Near Edge Structure (XANES) at the iron K edge of carbonmonoxy-myoglobin (MbCO) of a photoproduct and we have extracted the distribution functions g(H) of activation enthalpy barriers H for CO recombination for different conformational coordinates.
TDXAS study of the conformational landscape of MbCO
1999
Abstract
We have developed a new experimental approach, Temperature Derivative X-ray Absorption Spectroscopy (TDXAS), to measure the conformational landscape of a metalloprotein and how it depends on the selected conformational coordinate of the metal active site structure. We have recorded the temperature variation of a selected feature of the X ray Absorption Near Edge Structure (XANES) at the iron K edge of carbonmonoxy-myoglobin (MbCO) of a photoproduct and we have extracted the distribution functions g(H) of activation enthalpy barriers H for CO recombination for different conformational coordinates.File in questo prodotto:
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