Numerical analysis is implemented to investigate biological sample starting from Digital Holographic (DH) recording. The aim is to improve visualization and detection of cow spermatozoa. Digital holograms are recorded in the off-axis geometry where optical setup is a Mach-Zehnder interferometer. Then holograms are numerically manipulated to retrieve, besides the usual Quantitative Phase Map (QPM), Differential Interference Contrast (DIC) visualization. Furthermore, a new approach, named digital self-referencing holography, is described it's able to accomplish quantitative phase analysis especially useful for specimen flowing in microfluidic channels. © (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).
Detection and visualization improvement of spermatozoa cells by digital holography
Miccio L;Finizio A;Memmolo P;Paturzo M;Merola F;Coppola G;Ferraro P
2011
Abstract
Numerical analysis is implemented to investigate biological sample starting from Digital Holographic (DH) recording. The aim is to improve visualization and detection of cow spermatozoa. Digital holograms are recorded in the off-axis geometry where optical setup is a Mach-Zehnder interferometer. Then holograms are numerically manipulated to retrieve, besides the usual Quantitative Phase Map (QPM), Differential Interference Contrast (DIC) visualization. Furthermore, a new approach, named digital self-referencing holography, is described it's able to accomplish quantitative phase analysis especially useful for specimen flowing in microfluidic channels. © (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
