Genes coding far major albumins in Pisurn. Major Albumin (MA) of P. sativum and P. abyssiuicum are seed proteins composed by either two or one polypeptides which arc i?nherited as single rnendelian genes, Gcnomic blots of DNA prepared from CV. Feltharn First, line 993. (P. sativum, line 809 (P. abyssinicum) and from a mutant showing no major albumins (null albumin mutant) hybridised against a cDNA for rnajor albumin revealed the presence of a small polimorphic gene family. When analyzed at high stringency no restriction fragments omologous to the cDNA probe were detected in the null alburnin mutant, while some restriction fragments appear at moderately low stringency and the pattern indicate the possible presence of a large delection in the rnutant. Northern analyses of total RNA from the rnutant revealed no rnessages for MA proteins either at high or low stringcncy. One of the genes coding for MA has been isolated forn a genomic library constructed in EMBL3 and subcloned in either puC18 and M13 for sequence analysis.
Genes coding for major albumins in Pisum
Costa A;
1988
Abstract
Genes coding far major albumins in Pisurn. Major Albumin (MA) of P. sativum and P. abyssiuicum are seed proteins composed by either two or one polypeptides which arc i?nherited as single rnendelian genes, Gcnomic blots of DNA prepared from CV. Feltharn First, line 993. (P. sativum, line 809 (P. abyssinicum) and from a mutant showing no major albumins (null albumin mutant) hybridised against a cDNA for rnajor albumin revealed the presence of a small polimorphic gene family. When analyzed at high stringency no restriction fragments omologous to the cDNA probe were detected in the null alburnin mutant, while some restriction fragments appear at moderately low stringency and the pattern indicate the possible presence of a large delection in the rnutant. Northern analyses of total RNA from the rnutant revealed no rnessages for MA proteins either at high or low stringcncy. One of the genes coding for MA has been isolated forn a genomic library constructed in EMBL3 and subcloned in either puC18 and M13 for sequence analysis.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
