CNR Institutional Research Information System

Airwayepitheliumisemergingasaregulatorofinnateimmuneresponsestoa varietyofinsultsincludingcigarettesmoke.Themaingoalofthisstudywas to exploretheeffectsofcigarettesmokeextracts(CSE)inthetolllikereceptor (TLR) expressionandactivationinahumanbronchialepithelialcellline(16- HBE). CSEincreasedtheexpressionofTLR4andtheLPSbinding,theNFkB activation,thereleaseofIL-8andthechemotacticactivitytowardneutrophilsbut did notinducetheTLR2expressionandtheERK1/2activation.LPSincreasedthe expressionofTLR4andinducedbothNFkBandErk1/2activation.Thecombined exposureof16-HBEtoCSEandLPS,furtherincreasedIL-8releaseandthe chemotactic activitytowardneutrophils.Theseadditiveeffectswereassociated to ERKactivationratherthantoNFkBactivation.Furthermore,CSEdecreased the constitutiveIP-10releaseandcounteractedtheeffectofLPSininducing IP-10 release.Inconclusion,cigarettesmokecontributestotheaccumulationof neutrophils withintheairwaysofsmokersbyincreasingtheexpressionofTLR4 and bypreferentiallyorientatingTLR4activationtowardactivationofERKand IL-8 production. SupportedbyGlaxoSmithKline.

Cigarette smoke increases TLR4 expression and modifies LPS mediated responses in airway epithelial cells

E Pace;MFerraro;MR Bonsignore;MGjomarkaj;
2007

Abstract

Airwayepitheliumisemergingasaregulatorofinnateimmuneresponsestoa varietyofinsultsincludingcigarettesmoke.Themaingoalofthisstudywas to exploretheeffectsofcigarettesmokeextracts(CSE)inthetolllikereceptor (TLR) expressionandactivationinahumanbronchialepithelialcellline(16- HBE). CSEincreasedtheexpressionofTLR4andtheLPSbinding,theNFkB activation,thereleaseofIL-8andthechemotacticactivitytowardneutrophilsbut did notinducetheTLR2expressionandtheERK1/2activation.LPSincreasedthe expressionofTLR4andinducedbothNFkBandErk1/2activation.Thecombined exposureof16-HBEtoCSEandLPS,furtherincreasedIL-8releaseandthe chemotactic activitytowardneutrophils.Theseadditiveeffectswereassociated to ERKactivationratherthantoNFkBactivation.Furthermore,CSEdecreased the constitutiveIP-10releaseandcounteractedtheeffectofLPSininducing IP-10 release.Inconclusion,cigarettesmokecontributestotheaccumulationof neutrophils withintheairwaysofsmokersbyincreasingtheexpressionofTLR4 and bypreferentiallyorientatingTLR4activationtowardactivationofERKand IL-8 production. SupportedbyGlaxoSmithKline.
2007
Istituto di biomedicina e di immunologia molecolare - IBIM - Sede Palermo
Inglese
ERS Annual Congress
30 (suppl.51)
217s E1334
217s
Sì, ma tipo non specificato
September 15-19, 2007
Stockolm (Sweden)
9
none
Pace, E; Ferraro, Maria; Lsiena, ; Mmelis, ; Mjohnson, ; Bonsignore, Mr; Gbonsignore, ; Mgjomarkaj, ; Ammontalbano,
273
info:eu-repo/semantics/conferenceObject
04 Contributo in convegno::04.01 Contributo in Atti di convegno
04.01 Contributo in Atti di convegno
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.14243/2214
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