The natural contamination of sorghum and finger millet by toxigenic fungi and associated mycotoxins has been studied. All the tested sorghum and finger millet samples were found to be contaminated by Fusarium and Aspergillus species. Sorghum was considerably more likely to be contaminated by both genera than finger millet. Penicillium, Alternaria, Rhizopus and Epicoccum species were also present in both grains albeit at lower frequencies. Multimycotoxin analysis using LC-MS/MS revealed the contamination of sorghum and finger millet by 84 and 62 metabolites, respectively. The prevalence of major mycotoxins was lower than 15% in sorghum except zearalenone that occurred in one third of the samples at average level of 44 ?g/kg. In finger millet major mycotoxins occurred at a prevalence of 6-52% with zearalenone being the dominant and occurring at average level of 76 ?g/kg. Aflatoxins B1, B2, G1, G2 and M1 were detected in at least one sorghum sample while only aflatoxins B1 and G1 were present in finger millet samples. The average aflatoxins B1 and G1 concentrations in sorghum have been higher than European standards. But the level of B2, G2 and M1 in sorghum and that of B1 and G1 in finger millet have been lower. Apart from aflatoxin precursors and other Fusarium metabolites, a broad range of additional metabolites were detected in sorghum and finger millet.
Multimycotoxin analysis of sorghum (Sorghum bicolor L. Moench) and finger millet (Eleusine coracana L. Garten) from Ethiopia
2014
Abstract
The natural contamination of sorghum and finger millet by toxigenic fungi and associated mycotoxins has been studied. All the tested sorghum and finger millet samples were found to be contaminated by Fusarium and Aspergillus species. Sorghum was considerably more likely to be contaminated by both genera than finger millet. Penicillium, Alternaria, Rhizopus and Epicoccum species were also present in both grains albeit at lower frequencies. Multimycotoxin analysis using LC-MS/MS revealed the contamination of sorghum and finger millet by 84 and 62 metabolites, respectively. The prevalence of major mycotoxins was lower than 15% in sorghum except zearalenone that occurred in one third of the samples at average level of 44 ?g/kg. In finger millet major mycotoxins occurred at a prevalence of 6-52% with zearalenone being the dominant and occurring at average level of 76 ?g/kg. Aflatoxins B1, B2, G1, G2 and M1 were detected in at least one sorghum sample while only aflatoxins B1 and G1 were present in finger millet samples. The average aflatoxins B1 and G1 concentrations in sorghum have been higher than European standards. But the level of B2, G2 and M1 in sorghum and that of B1 and G1 in finger millet have been lower. Apart from aflatoxin precursors and other Fusarium metabolites, a broad range of additional metabolites were detected in sorghum and finger millet.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
