The preparation of a first bioglass material (45S5) was reported by L. Hench in [1,2], the particularity of this material being its ability of chemical bonding to bone. Since the first bioglass composition, many other formulations have been developed, and some of them also clinically tested. Nowadays, an increasing interest is focused on the third generation bioactive glass materials, providing tissue repair and regeneration through genes activation. It is known that various additions into bioactive glass-ceramic material may inhibit or accelerate its bone bonding ability, solubility, physicochemical and osteogenic gene expression properties, stimulating bone growth and angiogenesis. Trials to provide the compositional affinity with the natural bone tissue are realized by doping bioactive glass-ceramics with various elements. Mimicking composition of the original inorganic mineral phase of bone is a challenging issue in materials chemistry. Moreover, new compositions could be tailored in order to produce materials for specific clinical applications. Within this context, the sol-gel synthesis method is proposed as a potential tool for compositional tailoring, permitting the control of purity, surface area, porosity and homogeneity of material [3]. In this study, Pulsed Lased Deposition technique has been applied for coatings preparation. First successful trial and results regarding the innovative RKKP glass-ceramic coatings prepared by PLD have been recently reported by us in ref. [4]. PLD demonstrated several important advantages with respect to the other deposition procedures, such as the congruent transfer of target composition to coating, the possibility to control adherence, crystallinity, and surface roughness of the deposited coatings. The main scope of the present investigation is to find the suitable deposition conditions for the precise transfer of a sol-gel target of a selected composition into the coating and to test the biocompatibility and cell-friendly properties of these materials by seeding cells on them. For this purpose, various glass-ceramic materials are planned to be used. The characteristics of deposited coatings have been investigated by Scanning Electron Microscopy (SEM-EDS), Atomic Force Microscopy (AFM), Fourier Transform Infrared Spectroscopy (FTIR), Angular and Energy Dispersive X-Ray Diffraction (ADXRD and EDXRD). Vickers microhardness measurements of the composite film-substrate systems have been carried out, and the intrinsic hardness of films has been properly separated from the composite hardness, using the "law-of-mixtures" approach and taking into account the indentation size effect [5]."In vitro" tests are in progress, and preliminary results highlight the capability of human colon carcinoma cells (CaCo-2) to grow and proliferate on the investigated glass-ceramic materials.
New generation bioactive glass-ceramic materials for implant coating application in regenerative medicine.
JV Rau;FR Bertani;M Ledda;A Lisi
2014
Abstract
The preparation of a first bioglass material (45S5) was reported by L. Hench in [1,2], the particularity of this material being its ability of chemical bonding to bone. Since the first bioglass composition, many other formulations have been developed, and some of them also clinically tested. Nowadays, an increasing interest is focused on the third generation bioactive glass materials, providing tissue repair and regeneration through genes activation. It is known that various additions into bioactive glass-ceramic material may inhibit or accelerate its bone bonding ability, solubility, physicochemical and osteogenic gene expression properties, stimulating bone growth and angiogenesis. Trials to provide the compositional affinity with the natural bone tissue are realized by doping bioactive glass-ceramics with various elements. Mimicking composition of the original inorganic mineral phase of bone is a challenging issue in materials chemistry. Moreover, new compositions could be tailored in order to produce materials for specific clinical applications. Within this context, the sol-gel synthesis method is proposed as a potential tool for compositional tailoring, permitting the control of purity, surface area, porosity and homogeneity of material [3]. In this study, Pulsed Lased Deposition technique has been applied for coatings preparation. First successful trial and results regarding the innovative RKKP glass-ceramic coatings prepared by PLD have been recently reported by us in ref. [4]. PLD demonstrated several important advantages with respect to the other deposition procedures, such as the congruent transfer of target composition to coating, the possibility to control adherence, crystallinity, and surface roughness of the deposited coatings. The main scope of the present investigation is to find the suitable deposition conditions for the precise transfer of a sol-gel target of a selected composition into the coating and to test the biocompatibility and cell-friendly properties of these materials by seeding cells on them. For this purpose, various glass-ceramic materials are planned to be used. The characteristics of deposited coatings have been investigated by Scanning Electron Microscopy (SEM-EDS), Atomic Force Microscopy (AFM), Fourier Transform Infrared Spectroscopy (FTIR), Angular and Energy Dispersive X-Ray Diffraction (ADXRD and EDXRD). Vickers microhardness measurements of the composite film-substrate systems have been carried out, and the intrinsic hardness of films has been properly separated from the composite hardness, using the "law-of-mixtures" approach and taking into account the indentation size effect [5]."In vitro" tests are in progress, and preliminary results highlight the capability of human colon carcinoma cells (CaCo-2) to grow and proliferate on the investigated glass-ceramic materials.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
