The aim of this work was to investigate innovative reaction media such as ionic liquids for enzymatic phospholipids transformations in order to get a higher selectivity in the enzymatic transphosphatidyladon reactions of natural phosphatidylcholine catalyzed by phospholipase D (PLD) from Streptomyces PMF. The stability and the activity of the PLD in the ionic liquid [BMIM][PF6] were tested. This ionic liquid used as a cosolvent in the synthesis of phosphatidylserine lead surprisingly to the almost quantitative suppression of unwanted hydrolytic side reaction, thus providing a great improvement in the final product purification. (C) 2012 Elsevier B.V. All rights reserved.
Improvements in the enzymatic synthesis of phosphatidylserine employing ionic liquids
D'Arrigo Paola;Panzeri Walter;
2012
Abstract
The aim of this work was to investigate innovative reaction media such as ionic liquids for enzymatic phospholipids transformations in order to get a higher selectivity in the enzymatic transphosphatidyladon reactions of natural phosphatidylcholine catalyzed by phospholipase D (PLD) from Streptomyces PMF. The stability and the activity of the PLD in the ionic liquid [BMIM][PF6] were tested. This ionic liquid used as a cosolvent in the synthesis of phosphatidylserine lead surprisingly to the almost quantitative suppression of unwanted hydrolytic side reaction, thus providing a great improvement in the final product purification. (C) 2012 Elsevier B.V. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
