WALNET: Walnut Landscape Genetics In The Native Range Persian walnut is highly economically important species and is cultivated hroughout the temperate regions of the world for its high quality wood and edible nuts. Establishing the center(s) of diversity for the species, understanding its post glacial expansion events and shedding light to its history of domestication and dispersal by humans which began about 2000 yr ago in Eurasia can predict how walnut species will respond to possible future climate change scenarios. This project (WALNET) is designed to provide valuable information on how spatial landscape features influenced gene flow and promoted local adaptation in walnut. The specific objectives of the WALNET at USDA-HTIRC, Purdue University (USA) are (1) detect and analyze genetic structure of a large and unique collection of 105 Eurasian walnut populations collected over twenty years by CNR-IBAF (Porano, Terni, Italy) using neutral SSR markers developed for Juglans spp. at HTIRC (2) assess the nucleotide sequence diversity in a subset of phenotypically characterized walnut populations by functional SNPs markers in 500 adaptation-related genes. The Roche 454 sequencing platform will be applied for genotyping thousands of SNPs simultaneously in a large number of samples( 3) compare the genetic structure of walnut as inferred by SSR and SNPs markers and search signs of local adaptation of walnut populations (4) apply landscape genetic analysis with support of GIS technologies and spatial statistics tools to identify and quantify the effects of refugia during Last Glacial Maximum, post-glacial expansion, physical and climatic barriers, human domestication events, anthropological factors and connectivity between rural human communities on genetic differentiation of walnut populations. WALNET provides a special combination of spatial genomics and ecological expertise and is designed to fill a huge gap in the current understanding of the evolution of domesticated tree crops such as walnut species.
Walnut Landscape Genetics In The Native Range (WALNET)
Paola Pollegioni
2013
Abstract
WALNET: Walnut Landscape Genetics In The Native Range Persian walnut is highly economically important species and is cultivated hroughout the temperate regions of the world for its high quality wood and edible nuts. Establishing the center(s) of diversity for the species, understanding its post glacial expansion events and shedding light to its history of domestication and dispersal by humans which began about 2000 yr ago in Eurasia can predict how walnut species will respond to possible future climate change scenarios. This project (WALNET) is designed to provide valuable information on how spatial landscape features influenced gene flow and promoted local adaptation in walnut. The specific objectives of the WALNET at USDA-HTIRC, Purdue University (USA) are (1) detect and analyze genetic structure of a large and unique collection of 105 Eurasian walnut populations collected over twenty years by CNR-IBAF (Porano, Terni, Italy) using neutral SSR markers developed for Juglans spp. at HTIRC (2) assess the nucleotide sequence diversity in a subset of phenotypically characterized walnut populations by functional SNPs markers in 500 adaptation-related genes. The Roche 454 sequencing platform will be applied for genotyping thousands of SNPs simultaneously in a large number of samples( 3) compare the genetic structure of walnut as inferred by SSR and SNPs markers and search signs of local adaptation of walnut populations (4) apply landscape genetic analysis with support of GIS technologies and spatial statistics tools to identify and quantify the effects of refugia during Last Glacial Maximum, post-glacial expansion, physical and climatic barriers, human domestication events, anthropological factors and connectivity between rural human communities on genetic differentiation of walnut populations. WALNET provides a special combination of spatial genomics and ecological expertise and is designed to fill a huge gap in the current understanding of the evolution of domesticated tree crops such as walnut species.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
